Abstract
Cell suspension cultures were generated from transgenic tobacco plants expressing bean phenylalanine ammonia-lyase (PAL) or alfalfa cinnamate 4-hydroxylase (C4H) transgenes. PAL over-expressing cell lines exhibited high basal levels of PAL transcripts and enzymatic activity, but hardly exceeded wild-type cultures in the level of PAL activity obtained following elicitation. In contrast, expression of alfalfa C4H transcripts in unelicited cell suspension cultures did not result in a corresponding increase in C4H enzymatic activity. However, following elicitation, the level of C4H activity in over-expressing lines was significantly greater than in control lines.
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© 1999 Springer Science+Business Media Dordrecht
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Howles, P., Masoud, S.A., Blount, J.W., Rasmussen, S., Lamb, C., Dixon, R.A. (1999). Overexpression of L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase in tobacco cell suspension cultures. In: Altman, A., Ziv, M., Izhar, S. (eds) Plant Biotechnology and In Vitro Biology in the 21st Century. Current Plant Science and Biotechnology in Agriculture, vol 36. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4661-6_69
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DOI: https://doi.org/10.1007/978-94-011-4661-6_69
Publisher Name: Springer, Dordrecht
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