Abstract
On the initial attempt to make a chicken monoclonal antibody, a chicken cell line deficient in thymidine kinase activity, HU3R27, had been used as a fusion partner(Nishinaka et al., 1989). Antigen specific antibody-producing hybridomas were initially obtained, but soon lost the ability to produce antibody in culture. In addition, the type of all secreted antibodies was IgM.
In the present study, four improved cell lines, R27H1, R27H2, R27H3 and R27H4 were obtained for stable production of monoclonal antibody. These cell lines were obtained by fusion of HU3R27 with immunized chicken spleen cells. Fusion of these cell lines with Keyhole limpet haemocyanin (KLH)-immunized chicken spleen cells resulted in antibody-producing hybridomas. Subcloned hybridomas secreted highly reactive IgG and weakly reactive IgM to KLH. Some of these hybridomas have shown stable antibody secretion for over 6 months.
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© 1992 Springer Science+Business Media Dordrecht
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Nishinaka, S., Asaoka, H., Suzuki, T., Matsuda, H. (1992). Production of chicken monoclonal antibody. In: Murakami, H., Shirahata, S., Tachibana, H. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2844-5_71
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DOI: https://doi.org/10.1007/978-94-011-2844-5_71
Publisher Name: Springer, Dordrecht
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