Abstract
Rat hepatocytes in primary culture have been used for studying growth and differentiation functions such as drug metabolism. In order to develop a culture system keeping high capacity of xenobiotics-responsiveness, effects of extracellular matrix (ECM) on gene expressions for cytochrome P-450 (P-450), 3-hydroxy-3-methylglutaryl CoA reductase (HMG-CoA reductase) and malic enzyme were investigated. The treatment of xenobiotics to animals induce HMG-CoA reductase and malic enzyme as well as cytochrome P-450s. The inductions of the expression of HMG-CoA reductase gene and malic enzyme gene by polychlorinated biphenyls (PCB) were observed in primary cultured rat hepatocytes. This showed that the effect of PCB on the gene expression relating lipid metabolism was direct to hepatocytes. The induction was observed only in hepatocytes cultured on Engelbreth-Holm-Swarm sarcoma basement membrane gel (EHS-gel), not on type I collagen (TIC), which is usually used for monolayer culture of hepatocytes. The induction of CYP2B1/2 (cytochrome P-450b/e) gene expression was also observed only in the cells cultured on EHS-gel. These results demonstrated that the culture on EHS-gel was better than the usual monolayer culture for investigating the effect of xenobiotics.
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References
Pasco, D.S., Boyum, K.W., Merchant, S.N., Chalberg, S.C. and Fagan, J.B. (1988) Transcription and post-transcriptional regulation of the genes encoding cytochrome P-450c and P-450d in vivo and in primary hepatocyte cultures’, J. Biol. Chem., 263, 8671–8876.
Enat, R., Jefferson, D.M., Ruiz-Opazo, N., Gatmaitan, Z., Leinwand, L.A. and Reid, L.M. (1984) ‘Hepatocyte proliferation in vitro: Its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix’, Proc. Natl. Acad. Sci. USA, 81, 1411–1415.
Xanthopoulos, K.G., Mirkovitch, J., Decker, T., Kuo, C.F., and Darnell, J. J.E. (1989) ‘Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP’, Proc. Natl. Acad. Sci. USA, 86,4117–4121.
Bissell, D.M., Arenson, D.M., Maher, J.J. and Roll, F.J. (1987) ‘Support of cultured hepatocytes by a laminin-rich gel’, J. Clin. Invest., 79, 801–812.
Schuetz, E.G., Li, D., Omiecinski, C.J., Muller-Eberhard, U., Kleinman, H.K., Elswick, B. and Guzelian, P.S. (1988) ‘Regulation of gene expression in adult rat hepatocytes cultured in a basement membrane matrix’, J. Cell. Physiol., 134, 309–323.
Oda, H., Matsushita, N., Hirabayashi, A., and Yoshida, A. (1990) ‘Hyperlipoproteinemia in rats fed polychlorinated biphenyls’, J. Nutr. Sci. Vitaminol., 36, 117–122.
Oda, H., Yamashita, K., Sasaki, S., Horio, F., and Yoshida, A. (1987) ‘Long-term effects of dietary polychlorinated biphenyl and high level of vitamin E on ascorbic acid and lipid metabolism in rats’, J. Nutr., 117, 1217–1223.
Jenke, H.S. (1985) ‘Polychlorinated biphenyls interfere with the regulation of hydroxymethylglutaryl-coenzyme A reductase activity in rat liver via enzyme-lipid interaction and at the transcriptional level’, Biochim. Biophys. Acta, 837, 85–93.
Hitomi, Y., Wakayama, M., Oda, H., and Yoshida, A. (1993) ‘Liver-specific induction of NADPH-generating enzymes by polychlorinated biphenyls in rats’, Biosci. Biotech. Biochem. 57, 1134–1136.
Nagaoka, S., Kamuro, H., Oda, H., and Yoshida, A. (1991) ‘Effects of polychlorinated biphenyls on cholesterol and ascorbic acid metabolism in primary cultured rat hepatocytes’, Biochem. Pharmacol., 41, 1259–1261.
Chomozynski, P. and Sacchi, N. (1978) ‘Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction’, Anal. Biochem., 162, 156–159.
Fujii-Kuriyama, Y., Mizukami, Y., Kawajiri, K., Sogawa, K., and Muramatsu, M. (1982) ‘Primary structure of a cytochrome P-450: coding nucleotide sequence of phenobarbital-inducible cytochrome P-450 cDNA from rat liver’, Proc. Natl, Acad. Sci. USA, 79, 2793–2797.
Chin, D.J., Gil, G., Russell, D.W., Liscum, L., Luskey, K.L., Basu, S. K., Okayama, H., Berg, P., Goldstein, J.L., and Brown, M.S. (1984) ‘Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum’, Nature, 308, 613–617.
Dozin, B., Rall, J.E., and Nikodem, V.M. (1986). ‘Tissue-specific control of rat malic enzyme activity and messenger RNA levels by a high carbohydrate diet’, Proc. Natl. Acad. Sci. USA, 83, 4705–4709.
Horiuchi, K., Tajima, S., Menju, M., and Yamamoto, A. (1989) ‘Structure and expression of mouse apolipoprotein E gene’, J. Biochem., 106, 98–103.
Yoshimoto, K., Nakamura, T., and Ichihara, A. (1983) ‘Reciprocal effects of epidermal growth factor on key lipogenic enzymes in primary cultures of adult rat hepatocytes’ J. Biol. Chem., 258, 12355–12360.
DiPersio, C.M., Jackson, D.A., and Zaret, K. S. (1991) ‘The extracellular matrix coordinately modulates liver transcription factors and hepatocyte morphology’, Mol. Cell. Biol., 11, 4405–4414.
Jackson, D.A., Rowader, K.E., Stevens, K., Jiang, C, Milos, P., and Zaret, K.S. (1993) ‘Modulation of liver-specific transcription by interactions between hepatocyte nuclear factor 3 and nuclear factor 1 binding DNA in close apposition’, Mol. Cell. Biol., 13, 2401–2410.
Caron, J.M. (1990) ‘Induction of albumin gene transcription in hepatocytes by extracellular matrix proteins’, Mol. Cell. Biol., 10, 1239–1243.
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Oda, H., Suzuki, Y., Shibata, T., Hitomi, Y., Yoshida, A. (1994). Induction of HMG-CoA reductase and malic enzyme gene expressions by polychlorinated biphenyls in rat hepatocytes cultured on EHS-gel, not on Type I collagen. In: Kobayashi, T., Kitagawa, Y., Okumura, K. (eds) Animal Cell Technology: Basic & Applied Aspects. The Sixth International Meeting of Japanese Association for Animal Cell Technology JAACT’93, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0848-5_74
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DOI: https://doi.org/10.1007/978-94-011-0848-5_74
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