Abstract
Polystyrene culture dishes and polystyrene microcarriers were coated with two different peptides — Pronectin-F and polylysine, alone and in combination. Each of the two peptides alone supported cell attachment but cell spreading occurred only on Pronectin-F. When the two peptides were used in combination, the effect was synergistic. Attachment and spreading occurred at lower concentrations of peptides when they were used in combination than when they were used individually.
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References
Ruoslahti E, Pierschbacher MD (1986). Arg-Gly-Asp: A versatile cell recognition signal. Cell. 44:517–518.
Varani J, Dame M, Beals TF, Wass JA (1983). Growth of three established cell lines on glass microcarriers. Biotech. Bioengineer 25:1359–1372.
Varani J, Sitrin RG, Hillegas WJ (1992). Expression of plasminogen activator and plasminogen activator inhibitor mRNA in human fibroblasts grown on different substrates. Cytotechnology 9:157–162.
Plantefaber LC and Hynes RO (1989). Changes in integrin receptors on oncogenically transformed cells. Cell 56: 281–290.
Ginsburg I (1987). Cationic polyelectrolytes: A new look at their possible role as opsinins, as stimulators of respiratory burst in leukocytes, in bacteriolysis and as modulators of immune complex disease. Inflammation 11:489–515.
Van Wezel AL (1967). Growth of cell strains and primary cells on microcarriers. Nature. 216:65–66.
Nielson V, Giaever I (1980). Biosilon; Optimal culture conditions and various research scale culture techniques. Dev. Biol. Stand. 46:131–136.
Obrenovitch A, Maintier C, Sene C (1982). Microcarrier culture of fibroblastic cells on modified trisacryl beads. Biol. Cell 46: 249–256.
Keese O, Giaever I (1983). Cell growth on liquid microcarriers. Science 219: 1448–1449.
Varani J, Fligiel SEG, Inman DR, Helmreich DL, Bendelow MJ, Hillegas WJ (1989) Substrate-dependent differences in production of extracellular matrix molecules by squamous carcinoma cells and diploid fibroblasts. Biotech. Bioengineer 33:1235–1241.
Larsson B and Litwin J (1987). The growth of polio virus in human diploid fibroblasts grown with cellulose microcarriers in suspension cultures. Dev. Biol. Stand. 66:385–390.
Mered B (1981). Propagation of poliovirus in microcarrier cultures of three monkey kidney cell lines. J. Biol. Stand. 9:137–145.
Florentine D, Shahar A, Mizrahi A (1985). Production of herpesvirus of turkeys in a microcarrier, culturing system — A new method for production of vaccine against Maraks disease. Dev. Biol. Stand. 60:421–430.
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© 1994 Springer Science+Business Media Dordrecht
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Varani, J., Hillegas, W.J. (1994). Polystyrene Microcarriers Coated with a Combination of Adhesion-Promoting Peptides. In: Kobayashi, T., Kitagawa, Y., Okumura, K. (eds) Animal Cell Technology: Basic & Applied Aspects. The Sixth International Meeting of Japanese Association for Animal Cell Technology JAACT’93, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0848-5_71
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DOI: https://doi.org/10.1007/978-94-011-0848-5_71
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4366-3
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