Abstract
We have characterized the engrafted human peripheral blood lymphocytes (PBL) and the production of human immunoglobulin (Ig) in the chimeric SCID mice reconstituted with human PBL. Human cells from healthy donors were injected into the peritoneal cavity. Human lymphocytes expressing CD45 molecules migrated to lymphoid tissues of the chimeric mice and proliferated rapidly in only four days. However, B cells could not be detected in the thymus. We established several human B cell lines from human PBL in lymphoid tissues of the immunized mice that generated human IgG with specific binding activity to immunogens.
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Uchibayashi, N., Shino, A., Ochi, T., Okada, M., Okubo, Y., Shiho, O. (1994). Establishment of Human B Cell Lines Producing Antigen-specific Antibodies through SCID Mice Reconstituted with Human Peripheral Blood Lymphocytes. In: Kobayashi, T., Kitagawa, Y., Okumura, K. (eds) Animal Cell Technology: Basic & Applied Aspects. The Sixth International Meeting of Japanese Association for Animal Cell Technology JAACT’93, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0848-5_7
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DOI: https://doi.org/10.1007/978-94-011-0848-5_7
Publisher Name: Springer, Dordrecht
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