Abstract
We characterized the antibody repertoire specific to a short peptide (F21-40) of β-lactoglobulin (β-LG) by analyzing VH genes of B cell hybridomas prepared from BALB/c mice immunized with peptide-KLH conjugates. In an attempt to characterize the antibody repertoire elicited by F21-40, we established an in vitro culture system to obtain B cell clones specific to F21-40 and devised a protocol to determine the VH gene sequences.
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© 1994 Springer Science+Business Media Dordrecht
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Takahashi, Y., Ametani, A., Totsuka, M., Kaminogawa, S. (1994). In vitro culture of primary B cells specific to a short peptide. In: Kobayashi, T., Kitagawa, Y., Okumura, K. (eds) Animal Cell Technology: Basic & Applied Aspects. The Sixth International Meeting of Japanese Association for Animal Cell Technology JAACT’93, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0848-5_45
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DOI: https://doi.org/10.1007/978-94-011-0848-5_45
Publisher Name: Springer, Dordrecht
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