Effect of Dissolved Oxygen Concentration on Growth and Production of Biomaterials by Animal Cell Culture

Abstract

In large-scale or high density mammalian cell culture for production of pharmaceutical proteins, oxygen deficiency is one of the serious problems to be solved. DO (dissolved oxygen) concentration is one of the most critical parameters in designing the bioreactors. Till now effective surface aeration systems such as caged aeration, recirculation, silicon rubber tubing, increasing head space gas pressure, and using pure oxygen instead of air have been widely developed, but effects of DO on cell growth and metabolism have not been investigated in detail. Most studies on effects of DO limited to hybridoma cell lines and so far the DO levels investigated are only limited to the range from 0% to 100% air saturation (0‣ 6.8 mg/L). In hyperoxia condition (above 100 % air saturation), the effect of DO has never been studied for even a single mammalian cell line.