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Analysis of tissue culture-derived variation in pea (Pisum sativum L.)—preliminary results

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The Methodology of Plant Genetic Manipulation: Criteria for Decision Making

Part of the book series: Developments in Plant Breeding ((DIPB,volume 3))

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Summary

The possibility of producing agronomically-useful somaclones via organogenesis and somatic embryogenesis from callus cultures of pea (Pisum sativum L.) was studied. Organogenic calli were induced from immature leaflets on MSB medium with NAA and BAP. Embryogenic calli were derived either from immature zygotic embryos (using 2,4-D) or from shoot apices (using picloram) of aseptically-germinated seedlings.

The seed progenies (T1 to T3-generation) of primary regenerants were grown in field conditions and their phenotypic variation was evaluated and compared with control, non-tissue culture-derived plant material. In addition, electrophoretic analyses of selected isoenzyme systems and total proteins have been done. The results do not show dramatic changes in qualitative and quantitative traits. The evaluation of at least two future generations (T4, T5) is planned.

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Abbreviations

BAP:

6-benzylaminopurine

IBA:

indole-3-butyric acid

MSB:

medium (mineral salts after Murashige & Skoog, 1962, vitamins after Gamborg et al., 1968)

NAA:

α-naphthalene-acetic acid, picloram - 4-amino-3,5,6-trichloro picolinic acid

2,4-D:

2,4-dichlorophenoxyacetic acid, ORG - organogenesis

SE:

somatic embryogenesis

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Authors and Affiliations

  1. OSEVA, Research Institute of Technical Crops & Legumes, 787 01, Šumperk, Czech Republic

    Miroslav Griga & Jaromír Stejskal

  2. Research Institute for Cattle Breeding, Rapotín 267, 787 13, Vikýřovice, Czech Republic

    Karel Beber

Authors
  1. Miroslav Griga
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  2. Jaromír Stejskal
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  3. Karel Beber
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Editor information

Editors and Affiliations

  1. Department of Plant Science, University College, Cork, Ireland

    Alan C. Cassells  & Peter W. Jones  & 

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Griga, M., Stejskal, J., Beber, K. (1995). Analysis of tissue culture-derived variation in pea (Pisum sativum L.)—preliminary results. In: Cassells, A.C., Jones, P.W. (eds) The Methodology of Plant Genetic Manipulation: Criteria for Decision Making. Developments in Plant Breeding, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0357-2_40

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  • DOI: https://doi.org/10.1007/978-94-011-0357-2_40

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4159-1

  • Online ISBN: 978-94-011-0357-2

  • eBook Packages: Springer Book Archive

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