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Protoplast technology for the breeding of top-fruit trees (Prunus, Pyrus, Malus, Rubus) and woody ornamentals

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The Methodology of Plant Genetic Manipulation: Criteria for Decision Making

Part of the book series: Developments in Plant Breeding ((DIPB,volume 3))

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Summary

Until recently, temperate fruit trees and woody ornamentals have been regarded as recalcitrant to biotechnological breeding approaches based on protoplasts. This however should no longer be the case, as procedures are now available, not only for the regeneration of complete plants from protoplasts of various tissues of such species, but also for the exploitation of protoplast technology for their genetic manipulation. This paper will examine the recent advances and state of the art in this domain, with particular attention to the use of protoplast technology as a novel tool in the breeding of rosaceous top-fruit tree species and woody ornamentals. Problems and their solutions within the context of regenerating plants from isolated protoplasts of stone (Prunus spp.), pome (Pyrus spp., Malus spp.) and small (Rubus spp.) fruits, and of several shrubby ornamental genotypes (Lonicera spp., Weigela spp., Forsythia spp., Cotoneaster spp.) will be addressed. Interspecific (Prunus spinosa + Prunus cerasifera) and intergeneric (Forsythia spp. + Syringa spp.) somatic hybridization within this group of species, as well as the use of protoplasts for host/pathogen interaction studies (Pyrus/Erwinia amylovora) will also be discussed.

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Authors and Affiliations

  1. Station d’ Amélioration des Espèces Fruitières et Ornementales, I.N.R.A. Centre d’ Angers, B.P. 57, 49071, Beaucouzé Cedex, France

    Sergio J. Ochatt & Estela M. Patat-Ochatt

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  1. Sergio J. Ochatt
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  2. Estela M. Patat-Ochatt
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  1. Department of Plant Science, University College, Cork, Ireland

    Alan C. Cassells  & Peter W. Jones  & 

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Ochatt, S.J., Patat-Ochatt, E.M. (1995). Protoplast technology for the breeding of top-fruit trees (Prunus, Pyrus, Malus, Rubus) and woody ornamentals. In: Cassells, A.C., Jones, P.W. (eds) The Methodology of Plant Genetic Manipulation: Criteria for Decision Making. Developments in Plant Breeding, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0357-2_34

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  • DOI: https://doi.org/10.1007/978-94-011-0357-2_34

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4159-1

  • Online ISBN: 978-94-011-0357-2

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