Abstract
Seedlings of Avena sativa were precultivated hydroponically at pH 4.1 and subsequently treated with solutions containing 0 or 400 µM Al. Electron microscopic and X-ray microanalytic (EDXA) investigations were carried out on root tips after 1 to 10 days of treatment. Root growth and mitotic activity decreased rapidly upon application of Al. The meristematic tissues of Al-treated roots showed enhanced vacuolation. The cells, however, remained intact after a longer period of Al-treatment and no alterations in ultrastructure (for example of the nucleus) were visible. The EDX analyses of bulk frozen hydrated tissues showed that Al was predominantly localised in the walls of the outermost cortex cells. One day after onset of Al-stress no intracellular Al was detectable. Even after 10 days with continuous Al-stress, only small amounts of the absorbed Al were localised within the cells. These results suggest that the plasma membrane is a very effective barrier for Al in oats. It is improbable that impairments of cytoplasmatic functions are primary effects of Al-intoxication.
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References
Barlow P W and Adam J S 1989 The response of the primary root meristem of Zea mays L. to various periods of cold. J. Exp. Bot. 40, 81–88.
Bennet R J, Breen C M and Fey M V 1987 The effects of aluminium on root cap function and root development in Zea mays L.. Environ. Exp. Bot. 27, 91–104.
Corradi M G, Levi M, Musetti R and Favali M A 1991 The effect of Cr(VI) on different inbred lines of Zea mays. I. Nuclei and cell cycle in the root tip tissue. Protoplasma 162, 12–19.
Davies K L, Davies M S and Francis D 1991 Zinc-induced vacuo-lation in root meristematic cells of Festuca rubra L. Plant Cell Environ. 14, 399–406.
Eleftheriou E P, Moustakas M and Fragiskos N 1993 Aluminate-induced changes in morphology and ultrastructure of Thinopy-rum roots. J. Exp. Bot. 44, 427–436.
Hasenstein K H and Evans M L 1988 Comparative effectiveness of metal ions in inducing curvature of primary roots of Zea mays. Plant Physiol. 86, 885–889.
Horst W J, Wagner A and Marschner H 1983 Effects of aluminium on root growth, cell division rate and mineral element contents in roots of Vigna unguiculata genotypes. Z. Pflanzenphysiol. 105, 435–444.
Huang C X and van Steveninck R F M 1990 Salinity induced structural changes in meristematic cells of barley roots. New Phytol. 115, 17–22.
Johnson P A and Bennet R J 1991 Aluminum tolerance of root cap cells. J. Plant Physiol. 137, 760–762.
Kinraide T B, Arnold R C and Baligar V C 1985 A rapid assay for aluminium phototoxicity at submicromolar concentrations. Physiol. Plant. 65, 245–250.
Kleinig H and Sitte P 1993 Zellbiologie, 3rd edition, Gustav Fischer Verlag, Stuttgart, New York.
Marienfeld S and Stelzer R 1993 X-ray microanalyses in Al-treated Avena sativa plants. J. Plant Physiol. 141, 569–573.
Matsumoto H 1988 Changes of the structure of pea chromatin by aluminum. Plant Cell Physiol. 29, 281–287.
Matsumoto H and Morimura S 1980 Repressed template activity of chromatin of pea roots treated by aluminum. Plant Cell Physiol. 21, 981–989.
Morimura S, Takahashi E and Matsumoto H 1978 Association of aluminium with nuclei and inhibition of cell division in onion (Allium cepa) roots. Z. Pflanzenphysiol. 88, 395–401.
Naidoo G, McD Steward M and Lewis R J 1978 Accumulation sites of Al in snapbean and cotton roots. Agron. J. 70, 489–492.
Ryan P R, Ditomaso J M and Kochian L V 1993 Aluminium toxicity in roots: an investigation of spatial sensitivity and the role of the root cap. J. Exp. Bot. 44, 437–446.
Stelzer R, Kuo J and Koyro H W 1988 Substitution of Na+ by K+ in tissues and root vacuoles of barley (Hordeum vulgare L. cv. Aramir). J. Plant Physiol. 132, 671–677.
Zhang G and Taylor G J 1989 Kinetics of aluminum uptake by excised roots of aluminum-tolerant and aluminum-sensitive cul-tivars of Triticum aestivum L. Plant Physiol. 83, 159–162
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Marienfeld, S., Lehmann, H., Stelzer, R. (1995). Ultrastructural investigations and EDX-analyses of Al-treated oat (Avena sativa) roots. In: Date, R.A., Grundon, N.J., Rayment, G.E., Probert, M.E. (eds) Plant-Soil Interactions at Low pH: Principles and Management. Developments in Plant and Soil Sciences, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0221-6_47
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DOI: https://doi.org/10.1007/978-94-011-0221-6_47
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