Abstract
We have fabricated a microchip Coulter counter on a quartz substrate, and have used it to detect individual colloidal particles with a sensitivity proportional to each particle s size. We demonstrate the ability of this device to distinguish colloids whose diameters differ by less than 10%. Furthermore, the device is sensitive to individual molecules of γ,-phage DNA, which is a first step towards the application of this technique to the size-based differentiation of single biological macromolecules.
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References
O. A. Saleh and L. L. Sohn, Submitted to Rev. Sei. Instrum.
W. H. Coulter, U. S. Patent No. 2,656,508, issued 20 Oct. 1953
R. W. DeBlois and C. P. Bean, Rev. Sei. Instrum. 41(7), 909 (1970)
E. C. Gregg and K. D. Steidley, Biophys. J. 5, 393 (1965)
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© 2001 Springer Science+Business Media New York
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Saleh, O.A., Sohn, L.L. (2001). A Quantitative Nanoscale Coulter Counter. In: Ramsey, J.M., van den Berg, A. (eds) Micro Total Analysis Systems 2001. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1015-3_19
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DOI: https://doi.org/10.1007/978-94-010-1015-3_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3893-5
Online ISBN: 978-94-010-1015-3
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