Abstract
A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP™) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple applied pressure control. The valve structure is designed to control the width and thickness of the sample. We examined a fluorescent sandwich immunoassay for human AFP (α-fetoprotein) as a preliminary experiment and fast immunoassay within 30 minutes compared to the conventional one was realized in the micro flow cell.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this paper
Cite this paper
Honda, N., Shoji, S., Isomura, M., Ashihara, Y., Akahori, K., Sato, H. (2001). Fabrication of Micro Flow Cell for Multi-Analyte Sandwich Immunoassay Using Teflon Micro Check Valves. In: Ramsey, J.M., van den Berg, A. (eds) Micro Total Analysis Systems 2001. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1015-3_177
Download citation
DOI: https://doi.org/10.1007/978-94-010-1015-3_177
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3893-5
Online ISBN: 978-94-010-1015-3
eBook Packages: Springer Book Archive