Abstract
In support of our high throughput parallel peptide synthesis, which produces over 1000 synthetic peptide ligands per month, we have developed a high throughput method for validation and quantitation of synthetic peptides. This method includes automated LC-MS analysis, data processing, and sample purification steps. We were able to achieve high throughput while retaining sufficient sensitivity and resolution to accomplish characterization. At both analytical and semi-prep scale, data analysis and sample tracking were automated using a blend of proprietary and manufacturers’ software.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Chen, L., James, M., Korenstein, B., Miller, B., Carter, J.M. (2001). High Throughput Validation and Quantitation of Synthetic Peptides via LC-MS. In: Lebl, M., Houghten, R.A. (eds) Peptides: The Wave of the Future. American Peptide Symposia, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0464-0_145
Download citation
DOI: https://doi.org/10.1007/978-94-010-0464-0_145
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3905-5
Online ISBN: 978-94-010-0464-0
eBook Packages: Springer Book Archive