Abstract
Electrophoresis features of large DNA in dense pillar array structures were studied. The sieving structures were all-quartz-made, 200 – 500nm in diameter, 5 jim in height, and fabricated by dry etching process employing high selective Ni mask. Electrophoresis velocities of DNA in the structure were measured by a fluorescence microscope. The separation ability of T4 and lambda in the pillar region was dependent on the pillar size. The ability was significantly enhanced at the boundary where the DNA entered into the dense pillar region from free solution region. These results shows the importance of pillar pattern design for the DNA separation, and the possibility of high performance separation by optimizing the pillar size and the boundary effect.
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References
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J. Han and H. G. Craighead, Separation of long DNA molecules in a microfabricated entropic trap array, Science, 288, pp. 1026–1029, (2000).
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© 2002 Springer Science+Business Media Dordrecht
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Tezuka, Y. et al. (2002). DNA Size Separation Employing Micro-Fabricated Monolithic Nano-Structure. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0295-0_71
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DOI: https://doi.org/10.1007/978-94-010-0295-0_71
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3952-9
Online ISBN: 978-94-010-0295-0
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