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Rapid Precolumn Reaction and Chiral Separation on an Integrated Microchip

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Micro Total Analysis Systems 2002

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Abstract

We have performed precolumn derivatization and chiral separation of D,L-amino acids on an integrated poly(dimethylsiloxane) (PDMS) microchip. Enantiomers of amino acids are labeled with o-phthaldialdehyde/2,3,4,6-tetra-O-acetyl-thio-β-D-glucopyranose (OPA/TATG) in the microfabricated reactor, and separated by micellar electrokinetic chromatography (MEKC) in an achiral environment. Optimized chiral separations are achieved within 100 s using a buffer consisting of 25 mM sodium borate (pH 10.0) and 15 mM sodium dodecyl sulfate (SDS). The resolutions of enantiomeric OPA/TATG-derivatized amino acids are in the range of 1.4 ~ 6.1.

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References

  1. K. W. Ro, K. Lim, H. Kim, J. H. Hahn, “Poly(dimethylsiloxane) microchip for precolumn reaction and micellar electrokinetic chromatography of biogenic amines”, Electrophoresis 23 (2002) 1129–1137.

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  2. A. Tiveston, S. Folestad, “Chiral o-phthaldialdehyde reagents for fluorogenic on-column labeling of D- and L-amino acids in micellar electrokinetic chromatography”, Electrophoresis 18 (1997) 970–977.

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  3. H. Wan, L. G. Blomberg, “Chiral separation of amino acids and peptides by capillary electrophoresis”, J. Chromatogr. A 875 (2000) 43–88.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Pohang University of Science and Technology, San 31 Hyoja-Dong, Pohang, 790-784, South Korea

    Kyung Won Ro, Ho Kim & Jong Hoon Hahn

Authors
  1. Kyung Won Ro
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  2. Ho Kim
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  3. Jong Hoon Hahn
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Editor information

Editors and Affiliations

  1. The University of Tokushima, Tokushima, Japan

    Yoshinobu Baba

  2. Waseda University, Tokyo, Japan

    Shuichi Shoji

  3. University of Twente, Enschede, The Netherlands

    Albert van den Berg

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© 2002 Springer Science+Business Media Dordrecht

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Ro, K.W., Kim, H., Hahn, J.H. (2002). Rapid Precolumn Reaction and Chiral Separation on an Integrated Microchip. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0295-0_184

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  • DOI: https://doi.org/10.1007/978-94-010-0295-0_184

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3952-9

  • Online ISBN: 978-94-010-0295-0

  • eBook Packages: Springer Book Archive

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