Abstract
Recent developments in microfabrication processes and surface modification chemistry and micro detection methods have driven new waves of advanced technologies in the field of biochips of protein or peptide arrays which become a powerful tools for diagnosis of diseases and on-site bio-analysis. Here, we synthesized HPQ-containing pentapeptides on a glass chip and used streptavidin as a model protein to study the biding assay since HPQ-containing pentapeptides are well known to bind streptavidin [1].
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
K.S. Lam, S.E. Salmon, E.M. Hersh, V.J. Hruby, W.M. Kazmierski, and R.J. Knapp, A new type of synthetic peptide library for identifying ligand-binding activity, Nature, Vol. 354, pp. 82–4(1991).
S. Singh-Gasson, R.D. Green, Y. Yue, C. Nelson, F. Blattner, M. R. Sussman, F. Cerrina, Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array, Nat. Biotechnol., Vol. 17, pp. 974–78 (1999).
YS. Lee and D.H. Kim, The spectrophotometric binding assay for HPQ-containing pentapeptides on polymer support by using ST-AP conjugate, Peptide 1996, Mayflower Scientific Ltd., Birmingham, UK, pp. 571–72 (1997).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Shin, DS. et al. (2002). Micro Peptide Array Synthesis on a Chip. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0295-0_152
Download citation
DOI: https://doi.org/10.1007/978-94-010-0295-0_152
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3952-9
Online ISBN: 978-94-010-0295-0
eBook Packages: Springer Book Archive