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Detection of Single Nucleotide Incoporation Using Pyrosequencing in a Microfluidic Device

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Micro Total Analysis Systems 2002

Abstract

In this study, detection of single nucleotide incorporation using the DNA pyrosequencing technique in a microfluidic flow-through device is demonstrated. Pyrosequencing is based on the detection of released pyrophosphate during DNA synthesis. The microfluidic platform allowed detection of single base incorporation, enabling base-by-base DNA sequencing. The miniaturization has lead to a 1000-fold reduction in reagent consumption compared to the present standard volume in pyrosequencing and will hopefully enable reading of longer DNA sequences.

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References

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Author information

Authors and Affiliations

  1. Department of Signals, Sensors and Systems, Royal Institute of Technology, SE-10044, Stockholm, Sweden

    Aman Russom, Wouter van der Wijngaart, Helene Andersson & Göran Stemme

  2. Department of Biotechnology, Pyrosequencing AB, SE-752 28, Uppsala, Sweden

    Hanno Ehring & Nigel Tooke

Authors
  1. Aman Russom
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  2. Wouter van der Wijngaart
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  3. Hanno Ehring
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  4. Nigel Tooke
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  5. Helene Andersson
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  6. Göran Stemme
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Editor information

Editors and Affiliations

  1. The University of Tokushima, Tokushima, Japan

    Yoshinobu Baba

  2. Waseda University, Tokyo, Japan

    Shuichi Shoji

  3. University of Twente, Enschede, The Netherlands

    Albert van den Berg

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© 2002 Springer Science+Business Media Dordrecht

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Russom, A., van der Wijngaart, W., Ehring, H., Tooke, N., Andersson, H., Stemme, G. (2002). Detection of Single Nucleotide Incoporation Using Pyrosequencing in a Microfluidic Device. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0295-0_103

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  • DOI: https://doi.org/10.1007/978-94-010-0295-0_103

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3952-9

  • Online ISBN: 978-94-010-0295-0

  • eBook Packages: Springer Book Archive

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