Abstract
Conventionally prepared antibodies have been used for many years as research tools and for diagnostic and therapeutic purposes. They have been useful reagents in many respects, although their application has sometimes been limited by their inherent biochemical properties. If an animal is immunized with an immunogenic substance, a whole range of predetermined cell clones belonging to the class of B-lymphocytes is activated, induced to proliferate, and triggered to synthesize antibodies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Köhler, G. and Milstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specifity.Nature,256, 495
Eisen, H. N. and Siskind, G. W. (1964). Variations in affinities of antibody during the immune response.Biochemistry,3, 996
Siskind, G. W. and Benacerraf, B. (1969). Cell selection of antigen in the immune response.Adv. Immunol,10, 1
Jarvis, M. R., Casperson, G. F., Kranz, D. M. and Voss, E. W. (1982). Affinity maturation of NZB and Balb/cV mice. Antifluorescyl response.Molec. Immunol,19, 525
Moyle, W. R., Anderson, D. M. and Ehrlich, P. H. (1983). A circular antibody-antigen complex is responsible for increased affinity shown by mixtures of monoclonal antibodies to human chorionic gonadotropin,J. Immunol,131, 1900
Thompson, R. J. and Jackson, A. P. (1984). Cyclic complexes and high avidity antibodies.TIBS,9, 1
Richards, F. F., Konigsberg, W. H., Rosenstein, R. W. and Varga, J. M. (1975). On the specificity of antibodies.Science,187, 130
Lane, D. and Koprowski, H. (1982). Molecular recognition and the future of monoclonal antibodies.Nature,296, 200
Lennox, E. S. (1983). What can we learn about molecular homologies from cross-reactions of monoclonal antibodies.Transpl Proc.,15, 45
Sperling, R., Francus, T. and Siskind, G. W. (1983). Degeneracy of antibody specificity.J. Immunol,31, 882
Berzofsky, J. A. and Schechter, A. N. (1981). The concepts of cross- reactivity and specificity in immunology.Molec. Immunol,18, 751
Haaijman, J. J., Deen, C., Kröse, C. J. M., Zijlstra, J. J., Coolen, J. and Radi, J. (1984). A jungle of pitfalls.Immunol Today,5, 56
Editor information
Rights and permissions
Copyright information
© 1985 MTP Press Limited
About this chapter
Cite this chapter
Albert, W.H.W. (1985). Monoclonal antibodies: advantages and disadvantages in production of test systems. In: Shinton, N.K. (eds) New Technologies in Clinical Laboratory Science. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4928-7_11
Download citation
DOI: https://doi.org/10.1007/978-94-009-4928-7_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8684-4
Online ISBN: 978-94-009-4928-7
eBook Packages: Springer Book Archive