Skip to main content

Monoclonal antibodies: advantages and disadvantages in production of test systems

  • Chapter
New Technologies in Clinical Laboratory Science

Abstract

Conventionally prepared antibodies have been used for many years as research tools and for diagnostic and therapeutic purposes. They have been useful reagents in many respects, although their application has sometimes been limited by their inherent biochemical properties. If an animal is immunized with an immunogenic substance, a whole range of predetermined cell clones belonging to the class of B-lymphocytes is activated, induced to proliferate, and triggered to synthesize antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Köhler, G. and Milstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specifity.Nature,256, 495

    Article  PubMed  Google Scholar 

  2. Eisen, H. N. and Siskind, G. W. (1964). Variations in affinities of antibody during the immune response.Biochemistry,3, 996

    Article  PubMed  CAS  Google Scholar 

  3. Siskind, G. W. and Benacerraf, B. (1969). Cell selection of antigen in the immune response.Adv. Immunol,10, 1

    Google Scholar 

  4. Jarvis, M. R., Casperson, G. F., Kranz, D. M. and Voss, E. W. (1982). Affinity maturation of NZB and Balb/cV mice. Antifluorescyl response.Molec. Immunol,19, 525

    Article  CAS  Google Scholar 

  5. Moyle, W. R., Anderson, D. M. and Ehrlich, P. H. (1983). A circular antibody-antigen complex is responsible for increased affinity shown by mixtures of monoclonal antibodies to human chorionic gonadotropin,J. Immunol,131, 1900

    PubMed  CAS  Google Scholar 

  6. Thompson, R. J. and Jackson, A. P. (1984). Cyclic complexes and high avidity antibodies.TIBS,9, 1

    Google Scholar 

  7. Richards, F. F., Konigsberg, W. H., Rosenstein, R. W. and Varga, J. M. (1975). On the specificity of antibodies.Science,187, 130

    Article  PubMed  CAS  Google Scholar 

  8. Lane, D. and Koprowski, H. (1982). Molecular recognition and the future of monoclonal antibodies.Nature,296, 200

    Article  PubMed  CAS  Google Scholar 

  9. Lennox, E. S. (1983). What can we learn about molecular homologies from cross-reactions of monoclonal antibodies.Transpl Proc.,15, 45

    Google Scholar 

  10. Sperling, R., Francus, T. and Siskind, G. W. (1983). Degeneracy of antibody specificity.J. Immunol,31, 882

    Google Scholar 

  11. Berzofsky, J. A. and Schechter, A. N. (1981). The concepts of cross- reactivity and specificity in immunology.Molec. Immunol,18, 751

    Article  CAS  Google Scholar 

  12. Haaijman, J. J., Deen, C., Kröse, C. J. M., Zijlstra, J. J., Coolen, J. and Radi, J. (1984). A jungle of pitfalls.Immunol Today,5, 56

    Article  CAS  Google Scholar 

Download references

Authors

Editor information

N. K. Shinton

Rights and permissions

Reprints and permissions

Copyright information

© 1985 MTP Press Limited

About this chapter

Cite this chapter

Albert, W.H.W. (1985). Monoclonal antibodies: advantages and disadvantages in production of test systems. In: Shinton, N.K. (eds) New Technologies in Clinical Laboratory Science. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4928-7_11

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-4928-7_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8684-4

  • Online ISBN: 978-94-009-4928-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics