Abstract
A procedure involving the “Thymol-test” proposed by McGregor has been used for the indirect determination of HPLC response factors at 229 nm. Problems occuring by using this indirect method for calibration are discussed. Taking glucosinolates’ spectrum and the different approaches into account a comparision of all till now published HPLC response factors is made. The influence of different solvent systems and different HPLC equipment is considered and its importance for the establishment of internationally valid factors is discussed.
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© 1987 ECSC, EEC, EAEC, Brussels-Luxembourg
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Buchner, R. (1987). Approach to Determination of HPLC Response Factors for Glucosinolates. In: Wathelet, JP. (eds) Glucosinolates in Rapeseeds: Analytical Aspects. World Crops: Production, Utilization, Description, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3615-7_5
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DOI: https://doi.org/10.1007/978-94-009-3615-7_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-8118-4
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