Abstract
Two in vitro procedures are being tested for screening trembling aspen for resistance to Hypoxylon canker: (1) tissue culture response to fungal culture metabolites and (2) ascospore inoculation of moisture stressed plantlets. In the first procedure, the sensitivity of five clones to the acidic ethyl acetate (AEA) fraction of partially purified culture filtrate was determined by comparing callus growth on ACM medium containing the AEA fraction with that of a control containing non-inoculated culture filtrate. Treatments of 0.1x and 1.0x AEA concentrations were inhibitory compared with controls. The clones varied considerably in callus growth and callus inhibition, with the inhibition relative to growth of the control directly related to that growth. Correlations of the resistance responses of 10 year old ramets to artificial inoculations with mycelial-infested grain show a moderate positive correlation of both callus growth of the control and callus inhibition with canker length and with incidence of branch death.
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© 1988 Kluwer Academic Publishers
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Valentine, F., Baker, S., Belanger, R., Manion, P., Griffin, D. (1988). Screening for Resistance to Hypoxylon Mammatum in Populus Tremuloides Callus and Micropropagated Plantlets. In: Ahuja, M.R. (eds) Somatic Cell Genetics of Woody Plants. Forestry Sciences, vol 30. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2811-4_22
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DOI: https://doi.org/10.1007/978-94-009-2811-4_22
Publisher Name: Springer, Dordrecht
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