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Forms and nature of organic N in soil

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Nitrogen Economy in Tropical Soils

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 69))

Abstract

Most of the N in surface soils occurs in organic forms. The organic N in soil plays a key role in plant nutrition and soil fertility through its effects on microbial activity and nutrient availability. Typically, about one-third of the fertilizer N applied to temperate-zone soils is immobilized and retained in organic forms at the end of the growing season. A significant portion of this newly immobilized N is no more available to microorganisms and plants than the native humus N. Stabilization processes, probably involving polymerization of amino compounds and polyphenols, result in incorporation of N into humic substances with a concurrent reduction in N availability. This paper presents an account of the forms and nature of organic N in soil, emphasizing possible formation pathways, chemical characterization of humic substances through conventional and solid-state techniques, and the fate and composition of newly immobilized N in soil.

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References

  • Ahmad ZY, Yahiro HK and Harada T (1973) Factors affecting immobilization and release of nitrogen in soil and chemical characteristics of the nitrogen newly immobilized. IV. Soil Sci Plant Nutr (Tokyo) 19: 287–298

    CAS  Google Scholar 

  • Aldag RW (1977) Relations between pseudo-amide nitrogen and humic acid nitrogen released under different hydrolysis conditions. In: Soil organic matter studies, pp 293–299. Int Atomic Energy Agency, Vienna, Austria

    Google Scholar 

  • Allen AL, Stevenson FJ and Kurtz LT (1973) Chemical distribution of fertilizer nitrogen in soil as revealed by nitrogen-15 studies. J Environ Qual 2: 120–124

    Article  CAS  Google Scholar 

  • Amalfitano C, Pignalosa V, Auriemma L and Ramunni A (1992) The contribution of lignin to composition of humic acids from a wheat-straw amended soil during 3 years of incubation in pots. J Soil Sci 43: 495–504

    Article  CAS  Google Scholar 

  • Anderson DW, Paul EA and St Arnaud RJ (1974) Extraction and characterization of humus with reference to clay-associated humus. Can J Soil Sci 54: 317–323

    Article  CAS  Google Scholar 

  • Baldcock JA, Currie CJ and Oades JM (1991) Organic matter as seen by solid state 13C NMR and pyrolysis tandem mass spectrometry. In: Wilson WS (ed) Advances in soil organic matter research: The impact on agriculture and the environment, pp 45–60. Wiltshire, England: Redwood Press Ltd

    Google Scholar 

  • Batsula AA and Krupskiy NK (1974) The forms of nitrogen in humus substances of some virgin and developed soil of the left bank of the Ukraine. Sov Soil Sci 6: 456–462

    Google Scholar 

  • Benzing-Purdie L, Cheshire MV, Williams BL, Ratcliffe CI, Rip-meester JA and Goodman BA (1992) Interactions between peat and sodium acetate, ammonium sulphate, urea or wheat straw during incubation studied by 13C and 15N NMR spectroscopy. J Soil Sci 43: 113–125

    Article  CAS  Google Scholar 

  • Benzing-Purdie LM, Cheshire MV, Williams BL, Sparling GP, Ratcliffe CI and Ripmeester JA (1986) Fate of [15N] glycine in peat as determined by 13C and 15N CP-MAS NMR spectroscopy. J Agric Food Chem 34: 170–176

    Article  CAS  Google Scholar 

  • Benzing-Purdie L, Ripmeester JA and Preston CM (1983) Elucidation of the nitrogen forms in melanoidins and humic acid by nitrogen-15 cross polarization-magic angle spinning nuclear magnetic resonance spectroscopy. J Agric Food Chem 31: 913–915

    Article  CAS  Google Scholar 

  • Beyer L, Schulten H-R, Fruend R and Irmler U (1993) Formation and properties of organic matter in a forest soil, as revealed by its biological activity, wet chemical analysis, CPMAS 13C NMR spectroscopy and pyrolysis-field ionization mass spectrometry. Soil Biol Biochem 25: 587–596

    Article  CAS  Google Scholar 

  • Biederbeck VO and Paul EA (1973) Fractionation of soil humate with phenolic solvents and purification of the nitrogen rich portion with polyvinyl-pyrrolidone. Soil Sci 115: 357–366

    Article  CAS  Google Scholar 

  • Bracewell JM, Haider K, Larter SR and Schulten H-R (1989) Thermal degradation relevent to structural studies of humic substances. In: Hayes MHB et al. (eds) Humic substances II: In search of structure, pp 181–222. New York: Wiley

    Google Scholar 

  • Bremner JM (1955) Studies on soil humic acids: I. J Agr Sci 46: 247–256

    Article  CAS  Google Scholar 

  • Cheshire MV, Cranwell PA, Falshaw CP, Floyd AJ and Haworth RD (1967) Humic acid: II. Structure of humic acids. Tetrahedron 23: 1669–1682

    Article  CAS  Google Scholar 

  • Chichester FW (1970) Transformations of fertilizer nitrogen in soils. II. Total and 15N-labelled nitrogen of soil organo-mineral sedimentation fractions. Plant Soil 33: 437–456

    Article  CAS  Google Scholar 

  • Dehorter B, Kontchou CY and Blondeau R (1992) 13C NMR spectroscopic analysis of soil humic acids recovered after incubation with some white rot fungi and actinomycetes. Soil Biol Biochem 24: 667–673

    Article  CAS  Google Scholar 

  • Filip Z, Haider K, Beutelspacher H and Martin JP (1974) Comparisons of IR spectra from melanins of microscopic soil fungi, humic acids and model phenol polymers. Geoderma 11: 37–52

    Article  CAS  Google Scholar 

  • Führ F and Sauerbeck D (1968) Decompostion of wheat straw in the field as influenced by cropping and rotation. In: Isotopes and radiation in soil organic matter studies, pp 241–250. Tech Meeting, FAO/IAEA, Vienna, Austria

    Google Scholar 

  • Gamzikov GP (1981) Fertilizer nitrogen transformation: An interaction with West-Siberian soils. In: Dutil P and Jacquin F (eds) Colloque: humus-azote, pp 194–197. Station de Science du Sol, INRA, Charlons-Sar-Marne, France

    Google Scholar 

  • Goh KM and Edmeades DC (1979) Distribution and partial characterisation of acid hydrolyzable organic nitrogen in six New Zealand Soils. Soil Biol Biochem 11: 127–132

    Article  CAS  Google Scholar 

  • Griffith SM, Sowden FJ and Schnitzer M (1976) The alkaline hydrolysis of acid-resistant soil and humic acid residues. Soil Biol Biochem 8: 529–531

    Article  CAS  Google Scholar 

  • Haider K, Gröblinghoff F-F, Beck T, Schulten H-R, Hempfling R and Lüdermann HD (1991) Influence of soil management practices on the organic matter structure and the biochemical turnover of plant residues. In: Wilson WS (ed) Advances in soil organic matter research: The impact on agriculture and the environment, pp 79–91. Wiltshire, England: Redwood Press Ltd.

    Google Scholar 

  • Haworth RD (1971) The chemical nature of humic acid. Soil Sci 111:71–79

    Article  CAS  Google Scholar 

  • Hayes MHB (1991) Concepts of the origins, composition, and structures of humic substances. In: Wilson WS (ed) Advances in soil organic matter research: The impact on agriculture and the environment, pp 3–32. Wiltshire, England: Redwood Press Ltd

    Google Scholar 

  • He X-T, Stevenson FJ, Mulvaney RL and Kelley KR (1988) Incorporation of newly immobilized 15N into stable organic forms in soil. Soil Biol Biochem 20: 75–81

    Article  CAS  Google Scholar 

  • He X-T, Stevenson FJ, Mulvaney RL and Kelley KR (1988) Extraction of newly immobilized 15N from an Illinois Mollisol using aqueous phenol. Soil Biol Biochem. 20: 857–862

    Article  CAS  Google Scholar 

  • Hempfling R, Zech W and Schulten H-R (1988) Chemical composition of the organic matter in forest soils: 2. Moder profile. Soil Sci 146: 262–276

    Article  CAS  Google Scholar 

  • Hopkins DW and Shiel RS (1991) Spectroscopic characterization of organic matter from soil with mor and mull humus forms. In: Wilson WS (ed) Advances in soil organic matter research: The impact on agriculture and the environment, pp 71–78. Wiltshire, England: Redwood Press Ltd

    Google Scholar 

  • Ivarson KC and Schnitzer M (1979) The biodegradability of the “unknown” soil-nitrogen. Can J Soil Sci 59: 59–67

    Article  CAS  Google Scholar 

  • Jenkinson DS (1965) Studies on the decomposition of plant material in soil I.J. Soil Sci. 16: 104–115

    Article  CAS  Google Scholar 

  • Kai H, Ahmad Z and Harada T (1973) Factors affecting immobilization and release of nitrogen in soil and chemical characteristics of the nitrogen newly immobilized. III. Soil Sci Plant Nutr (Tokyo) 19: 275–286

    CAS  Google Scholar 

  • Kang KS and Felbeck Jr GT (1965) A comparison of the alkaline extract of tissues of Aspergillus niger with humic acids from three soils. Soil Sci 99: 175–181

    Article  CAS  Google Scholar 

  • Keeney DR (1982) Nitrogen — Availability indices. In: Page AL et al. (eds) Methods of soil analysis, Part 2, Chemical and microbiological properties. Agronomy 9: 711–734. Madison, Wisconsin: Am Soc Agron.

    Google Scholar 

  • Kelley KR and Stevenson FJ (1985) Characterization and extractability of immobilized 15N from the soil microbial biomass. Soil Biol Biochem 17: 517–523

    Article  Google Scholar 

  • Kelley KR and Stevenson FJ (1987) Effects of carbon source on immobilization and chemical distribution of fertilizer N in soil. Soil Sci Soc Am J 51: 946–951

    Article  CAS  Google Scholar 

  • Khan SU and Sowden FJ (1972) Distribution of nitrogen in ful-vic acid fraction extracted from the black Solonetzic and black Chernozemic soils of Alberta. Can J Soil Sci 52: 116–118

    Article  Google Scholar 

  • Kögel-Knabner I, Hatcher PG and Zech W (1991) Chemical structural studies of forest soil humic acids: Aromatic carbon fraction. Soil Sci Soc Am J 55: 241–247

    Article  Google Scholar 

  • Krosshavn, M, Kögel-Knabner I, Southon TE and Steinnes E (1992) The influence of humus fractionation on the chemical composition of soil organic matter studied by solid-state 13C NMR. J Soil Sci 43: 473–483

    Article  CAS  Google Scholar 

  • Krosshavn M, Southon TE and Steinnes E (1992) The influence of vegetational origin and degree of humification of organic soils on their chemical composition, determined by solid-state 13C NMR. J Soil Sci 43: 485–493

    Article  CAS  Google Scholar 

  • Ladd JN and Jackson RB (1982) Biochemistry of ammonification. In: Stevenson F J (ed) Nitrogen in agricultural soils. Agronony 22: 173–228. Madison, Wisconsin: Am. Soc Agron

    Google Scholar 

  • Leinweber P and Schulten H-R (1993) Dynamics of soil organic matter studied by pyrolysis-field ionization mass spectrometry. J Anal Appl Pyrolysis 25: 123–136

    Article  CAS  Google Scholar 

  • Leirós, MC, Gil-Sotres F, Ceccanti B, Trasar-Cepeda MC and González-Sangregorio MV (1993) Humification in reclaimed open-cast lignite mine spoils. Soil Biol Biochem. 25: 1391–1397

    Article  Google Scholar 

  • Malcolm, RL (1989) Application of solid-state 13C NMR spectroscopy to geochemical studies of humic substances. In: Hayes MHB et al. (eds) Humic substances II: In search of structure, pp 339–372. New York: Wiley

    Google Scholar 

  • Malcolm RL and MacCarthy P (1990) The individuality of humic substances in diverse environments. In: Wilson WS (ed) Advances in soil organic matter research: The impact on agriculture and the environment, pp 23–34. Wiltshire, England: Redwood Press Ltd

    Google Scholar 

  • Malik KA and Haider K (1982) Decomposition of 14C-labelled melanoid fungal residues in a marginally sodic soil. Soil Biol Biochem 14: 457–460

    Article  CAS  Google Scholar 

  • McGill WB and Paul EA (1976) Fractionation of soil and 15N nitrogen to separate the organic and clay interactions of immobilized N. Can J Soil Sci 56: 203–212

    Article  CAS  Google Scholar 

  • McGill WB, Shields JA and Paul EA (1975) Relation between carbon and nitrogen turnover in soil. Organic fractions of microbial origin. Soil Biol Biochem 7: 57–63

    Article  CAS  Google Scholar 

  • Meuzelaar HLC, Haider K, Nagar BR and Martin JP (1977) Comparative studies of pyrolysis-mas s spectra of melanins, model phenolic polymers, and humic acids. Geoderma 17: 239–252

    Article  CAS  Google Scholar 

  • Oberländer HE and Roth K (1968) Transformations of 14C-labeled plant material in soils under field conditions. In: Isotopes and radiation in soil organic matter studies, pp 351–361. Tech meeting, FAO/IAEA Vienna, Austria

    Google Scholar 

  • Otsuki A and Hanya T (1967) Some precursors of humic acid in recent lake sediments suggested by infrared spectra. Geochim Cosmochim Acta 31: 1505–1515

    Article  CAS  Google Scholar 

  • Piccolo A, Campanella L and Petronio BM (1990) Carbon-13 nuclear magnetic resonance spectra of soil humic substances extracted by different mechanisms. Soil Sci Soc Am J 54: 750–756

    Article  CAS  Google Scholar 

  • Piccolo A, Nardi S and Concheri G (1992) Structural characteristics of humic substances as related to nitrate uptake and growth regulation in plant systems. Soil Biol Biochem 24: 373–380

    Article  CAS  Google Scholar 

  • Piper TJ and Posner AM (1968) On the amino acids found in humic acids. Soil Sci 106: 188–192

    Article  CAS  Google Scholar 

  • Preston CM, Newman RH and Rother P (1994) Using 13C CPMAS NMR to assess effects of cultivation on the organic matter of particle size fractions in a grassland soil. Soil Sci 157: 26–35

    Article  CAS  Google Scholar 

  • Rosell RA, Salfeld JC and Sochtig H (1978) Organic components in Argentine soils: 1. Nitrogen distribution in soils and their humic acids. Ochimica 22: 98–105

    CAS  Google Scholar 

  • Rudelov YV and Prynanishnikov DN (1982) Dynamics of immobilized nitrogen. Soviet Soil Sci 14: 40–45

    Google Scholar 

  • Russell JD, Vaughan D, Jones D and Fraser AR (1983) An IR spectroscopic study of soil humin and its relationship to other soil humic substances and fungal pigments. Geoderma 29: 1–12

    Article  CAS  Google Scholar 

  • Saiz-Jimenez C (1983) The chemical nature of the melanins from Coprinus spp. Soil Sci 136: 65–74

    Article  CAS  Google Scholar 

  • Saiz-Jimenez C (1995) The chemical structure of humic substances: Recent advances. In: Piccolo A (ed) Humic substances in terrestrial ecosystems, (in press). Amsterdam, Elsevier Publ

    Google Scholar 

  • Schnitzer M (1985) Nature of nitrogen in humic substances. In: Aiken GR et al. (eds) Humic substances in soil, sediment, and water, pp 303–325. New York: Wiley-Interscience

    Google Scholar 

  • Schnitzer M (1991) Soil organic matter-the next 75 years. Soil Sci 151:41–58

    Article  Google Scholar 

  • Schnitzer M and Chan YK (1986) Structural characteristics of a fungal melanin and a soil humic acid. Soil Sci Soc Am J 50: 67–71

    Article  CAS  Google Scholar 

  • Schnitzer M, Ortiz de Serra MI and Ivarson K (1973) The chemistry of fungal humic acid-like polymers and of soil humic acids. Soil Sci Soc Am Proc 37: 229–236

    Article  CAS  Google Scholar 

  • Schnitzer M and Schulten H-R (1992) The analysis of soil organic matter by pyrolysis-field ionization mass spectrometry. Soil Sci Soc Am J 56: 1811–1817

    Article  CAS  Google Scholar 

  • Schulten H-R (1977) Pyrolysis-field ionization and field-desorption mass spectrometry of biomacromolecules, microorganisms, and tissue material. In: Jones CER and Cramers CA (eds) Analytical Pyrolysis, pp 17–28. Amsterdam: Elsevier Publ

    Google Scholar 

  • Schulten H-R (1987) Pyrolysis and soft ionization mass spectrometry of aquatic/terrestrial humic substances and soils. J Anal Appl Pyrolysis 12: 149–186

    Article  CAS  Google Scholar 

  • Schulten H-R and Hempfling R (1992) Influence of agricultural soil management on humus composition and dynamics: Classical and modern analytical techniques. Plant and Soil 142: 259–271

    Article  CAS  Google Scholar 

  • Schulten H-R and Leinweber P (1991) Influence of long-term fertilization with farmyard manure on soil organic matter: Characteristics of particle-size fractions. Biol Fertil Soils 12: 81–88

    Article  CAS  Google Scholar 

  • Schulten H-R and Leinweber P (1993) Pyrolysis-field ionization mass spectrometry of agricultural soils and humic substances: Effect of cropping systems and influence of the mineral matrix. Plant and Soil 151: 77–90

    Article  CAS  Google Scholar 

  • Schulten H-R and Schnitzer M (1993) A state of the art structural concept for humic substances. Naturwissenschaften 80: 29–30

    Article  CAS  Google Scholar 

  • Shields JA and Paul EA (1973) Decomposition of 14C-labelled plant material under field conditions. Can J Soil Sci 53: 297–306

    Article  CAS  Google Scholar 

  • Simonart P, Batistic L and Mayaudon J (1967) Isolation of protein from humic acid extracted from soil. Plant Soil 27: 153–161

    Article  CAS  Google Scholar 

  • Smith SJ, Chichester FW and Kissel DE (1978) Residual forms of fertilizer nitrogen in field soils. Soil Sci 125: 165–169

    Article  CAS  Google Scholar 

  • Sorge C, Schnitzer M and Schulten H-R (1993) In-source pyrolysisfield ionization mass spectrometry and Curie-point pyrolysisgas chromatography/mass spectrometry of amino acids in humic substances and soils. Biol Fertl Soils 16: 100–110

    Article  CAS  Google Scholar 

  • Sowden FJ and Schnitzer M (1967) Nitrogen distribution in illuvial organic matter. Can J Soil Sci 47: 111–116

    Article  CAS  Google Scholar 

  • Stanford G (1982) Assessment of soil nitrogen availability. In: Stevenson FJ (ed) Nitrogen in agricultural soils. Agronomy 22: 651–688. Madison, Wisconsin: Am. Soc Agron

    Google Scholar 

  • Stevenson FJ (1982) Humus Chemistry. New York: Wiley-Interscience

    Google Scholar 

  • Stevenson FJ (1982) Nitrogen — Organic forms. In: Page Al et al. (eds) Methods of soil analysis, Part 2, Chemical and microbiological properties. Agronomy 9: 625–641. Madison, Wisconsin: Am Soc Agron

    Google Scholar 

  • Stevenson FJ (1982) Organic forms of soil nitrogen. In: Stevenson FJ (ed) Nitrogen in agricultural soils. Agronomy 22: 67–122. Madison, Wisconsin: Am Soc Agron

    Google Scholar 

  • Stevenson FJ (1985) Cycles of soil: C, N, P, S, micronutrients. New York: Wiley-Interscience

    Google Scholar 

  • Stevenson FJ and Goh KM (1971) Infrared spectra of humic acids and related substances. Geochim Cosmochim Acta 35: 4714–83

    Article  Google Scholar 

  • Stevenson FJ and He X-T (1990) Nitrogen in humic substances as related to soil fertility. In: MacCarthy P et al. (eds) Humic substances in soil and crop sciences; Select readings, pp 91–109. Madison, Wisconsin: Am Soc Agron

    Google Scholar 

  • Stevenson FJ and Kelley KR (1985) Nitrogen transformations in soil: A perspective. In: Malik KA et al. (eds) Nitrogen and the environment, pp 7–26. Faisalabad, Pakistan: Nuclear Institute for Agriculture and Biology

    Google Scholar 

  • Su-Neng Z and Qi-Xiao W (1992) Nitrogen forms in humic substances. Pedosphere 4: 307–315

    Google Scholar 

  • Su-Neng Z, Qi-Xiao W, Li-Juan D and Shun-Ling W (1992) The nitrogen form of nonhydrolyzable residue of humic acid. Chinese Science Bulletin 37: 508–511

    Google Scholar 

  • Tan KH, Sihanonth P and Todd RL (1978) Formation of humic acid like compounds by the entomycorrhizal fungus, Pisolithus tinctorius. Soil Sci Soc Am J 42: 906–908

    Article  CAS  Google Scholar 

  • Tate III RL (1987) Soil organic matter. New York: Wiley-Interscience

    Google Scholar 

  • Tsutsuki K and Kuwatsuka S (1978) Chemical studies of soil humic acids: III. Soil Sci Plant Nutr 24: 29–38

    Google Scholar 

  • Wagner GH (1968) Significance of microbial tissue to soil organic matter. In: Isotopes and radiation in soil organic matter studies, pp 197–205. Tech. meeting, FAO/IAEA, Vienna, Austria

    Google Scholar 

  • Waksman SA (1932) Humus. Baltimore, Maryland: Williams and Wilkins

    Google Scholar 

  • Wilson MA (1989) Solid-state nuclear magnetic resonance spectroscopy of humic substances: Basic concepts and techniques. In: Hayes MHB et al. (eds) Humic substances II: In search of structure, pp 309–338. New York: Wiley

    Google Scholar 

  • Wilson MA (1990) Application of nuclear magnetic resonance spectroscopy to organic matter in whole soils. In: MacCarthy P et al. (eds) Humic substances in soil and crop sciences; Selected readings, pp 221–260. Madison, Wisconsin: Am Soc Agron

    Google Scholar 

  • Wojcik-Wojtikowiak D (1978) Nitrogen transformations in soil during humification of straw labeled with 15N. Plant Soil 49: 49–55

    Article  Google Scholar 

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Kelley, K.R., Stevenson, F.J. (1995). Forms and nature of organic N in soil. In: Ahmad, N. (eds) Nitrogen Economy in Tropical Soils. Developments in Plant and Soil Sciences, vol 69. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1706-4_1

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  • DOI: https://doi.org/10.1007/978-94-009-1706-4_1

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