Abstract
Studies of alkalophiles have led to the discovery of many new types of enzymes which exhibit unique properties in many respects. Work with these microorganisms, has led to the establishment of an ‘Alkaline Fermentation Process’. By this process, about 35 new kinds of enzymes were isolated and purified. Some of them were genetically investigated, and their genes were cloned and sequenced. One of the most important factors in the production of these alkaline enzymes is pH control of the culture media as well as other factors, such as carbon source or nitrogen source. For laboratory scale, the culture media commonly contain 1% sodium carbonate or sodium bicarbonate, which is usually sufficient to maintain the pH above 8 without readjustment during cultivation (Table 1).
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© 1990 Elsevier Science Publishers Ltd
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Horikoshi, K. (1990). Enzymes of Alkalophiles. In: Fogarty, W.M., Kelly, C.T. (eds) Microbial Enzymes and Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0765-2_8
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DOI: https://doi.org/10.1007/978-94-009-0765-2_8
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