Abstract
Some selected parameters of hemostasis that are currently thought to play a major role in thrombogenesis were evaluated in 5 patients homozygous for familial hypercholesterolemia (FH) before and 7 and 14 days after a single LDL-apheresis. The sensitivity of platelets to threshold concentrations of ADP was normal and it was not affected by LDL-apheresis. On the other hand, the sensitivity to arachidonic acid or collagen was abnormally high and this enhanced sensitivity was unchanged 7 and 14 days after the apheresis. Monocyte pro-coagulant activity (PCA) was normal before the apheresis and its values were little affected by the procedure. Likewise, the levels of plasminogen activator inhibitor (PAI) as well as the release by vessel walls of tissue plasminogen activator (tPA) the major phisiological activator of fibrinolysis were functionally and antigenicaliv normal before as well as 7 and 14 days after the apheresis. We conclude that the ability of LDL-apheresis to reduce the tendency to thrombosis of these patients is not associated with changes in these major parameters of hemostasis.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Goldstein, J.L., Brown, M.S., Familial hypercholesterolemia: A genetic defect in the low density lipoprotein receptor. New Engl. J. Med. 294: 1386–1390, 1976
Di Mimmo G, Mane in i M. Measuring Plasma Fibrinogen to Predict Stroke and Myocardial Infarction. Arteriosclerosis 1989, in Press
Di Minno G, Cerbone AM. The Antithrombotic Potential of the Vessel Wall. Evidence From Studies on Patients Prone to Venous Thrombosis. Haematologica, 73, 75–78, 1988
Yokoyama, S., Hayashi, R., Kikkava, T.. Tani, N., Takada, S., Hatanaka, K., Yamamoto, A., Specific sorbent of apolipoprotein B-containing lipoproteins for plasmapheresis: characterization and experimental use in hypercholesterolemic rabbits. Arteriosclerosis 4: 276–282, 1984
Boyum A 1976. Isolation of Lymphocytes, Granulocytes and Macrophages. Scand J. Immunol (Suppl)5: 9–15
Montemurro P, A Lattanzio, G. Chetta, L. Lupo, L. Caputi- Lambrenghi, M. Rubino, D. Giordano and N. Semeraro, 1985. Increased in vitro and in Vivo Generation of Pro-coagulanrt Activity ( Tissue Factor) by Mononuclear Phagocytes after Intralipid Infusion in Rabbits. Blood, 65, 1391–1395.
Declerck PJ, Alessi MC, Verstreken M, Kruithof EKO, Juhan-Vague I, Collen D: Measurement of Plasminogen Activator Inhibitor 1 in Biological Fluids with a Murine Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay. Blood 71, 220–225, 1988.
Colucci M, Paramo JA, Collen D. Generation in Plasma of a Fast-Acting Inhibitor of Plasminogen Activatoe in Response to Endotoxin Stimulation. J. Clin. Invest. 75, 818–824, 1985
Holvoet P, Cleemput H, Collen D: Assay of Human Tissue- Type Plasminogen Activator (tPA) With an Enzyme-Linked Immunosorbent Assay (ELISA) Based on Three Murine Monoclonal Antibodies to tPA. Thrombos. Haemostas. 54, 684–687, 1985.
Rijken DC, Collen D. Purification and Characterization of the Plasminogen Activator Secreted by Human Melanoma Cells in Culture. J. Biol. Chem. 256. 7035–7041, 1981.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Kluwer Academic Publishers
About this chapter
Cite this chapter
Di Minno, G. et al. (1990). The effect of LDL-apheresis on some hemostatic parameters in homozygous familial hypercholesterolemia. In: Descovich, G., Gaddi, A., Magri, G., Lenzi, S. (eds) Atherosclerosis and Cardiovascular Disease. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0731-7_36
Download citation
DOI: https://doi.org/10.1007/978-94-009-0731-7_36
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6814-7
Online ISBN: 978-94-009-0731-7
eBook Packages: Springer Book Archive