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Coupling of the PS2 Reaction Center to the O2-Evolving Center Requires a Very High Affinity Ca2+ Site

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Current Research in Photosynthesis

Abstract

Despite considerable research implicating Ca requirements for O2 evolution, controversy currently exists regarding the number of PS2 Ca site(s) and the reaction(s) affected by extraction/repletion. Ca analyses indicate 1–3 (1–3) and 1 Ca (4) are required for O2 evolution by higher plant and Synechococcus PS2, respectively. Several groups have indicated the S1→S2 is inhibited by Ca depletion (5–7) while others have indicated inhibition of the S3→S0 (8) and the S2→S3 (9) transitions. Such controversy might arise if >1 Ca are ligatea with differing affinities (1,10). Using spinach PS2 Core Complexes (≥2000 O2/Chl·h), we attempted to prepare Complexes containing <1, 1, 2 or 3 Ca/RC for analyses by EPR and O2 rate measurements.

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Author information

Authors and Affiliations

  1. University of Kentucky, Lexington, KY, USA

    Katerina Kalosaka & G. Cheniae

  2. Yale University, New Haven, CT, USA

    W. F. Beck & G. Brudvig

Authors
  1. Katerina Kalosaka
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  2. W. F. Beck
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  3. G. Brudvig
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  4. G. Cheniae
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Stockholm, Stockholm, Sweden

    M. Baltscheffsky

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© 1990 Springer Science+Business Media New York

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Kalosaka, K., Beck, W.F., Brudvig, G., Cheniae, G. (1990). Coupling of the PS2 Reaction Center to the O2-Evolving Center Requires a Very High Affinity Ca2+ Site. In: Baltscheffsky, M. (eds) Current Research in Photosynthesis. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0511-5_164

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  • DOI: https://doi.org/10.1007/978-94-009-0511-5_164

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6716-4

  • Online ISBN: 978-94-009-0511-5

  • eBook Packages: Springer Book Archive

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