Abstract
Ever since Knudson [3] developed a nonsymbiotic in vitro culture method for germination of orchid seeds, tissue (in vitro) culture has played an important and highly significant role in the cultivation, propagation, breeding and preservation of orchid species and hybrids. The purpose of this chapter is to present the procedures in detail for clonal propagation of orchids as successfully practiced in our laboratory.
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References
Arditti J (1977) Clonal propagation of orchids by means of tissue culture — A manual. In: Orchid Biology Reviews and Perspectives, I ( J Arditti, ed.) pp. 203–293 Cornell Univ. Press, Ithaca, NY
Goh CJ (1990) Orchids, Monopodials. In: Handbook of Plant Cell Culture 5 ( Ammirato PV, Evans DA, Sharp WP, Bajaj YPS, eds.) pp. 598–637 McGraw-Hill Inc. NY
Knudson L (1946) A new nutrient solution for germination of orchid seed. Amer. Orchid Soc. Bull. 15: 214–217.
Morel GM (1960) Producing virus-free Cymbidiums. Amer. Orchid Soc. Bull. 29: 495–497.
Rao AN (1977) Tissue culture in the orchid industry. In: Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture ( Reinert J & Baja] YPS, eds.) pp. 44–69. Springer-Verlag, NY
Sagawa Y (1990) Orchids, Other Considerations. In: Handbook of Plant Cell Culture 5 ( Ammirato PV, Evans DA, Sharp WP, Bajaj YPS, eds.) pp. 638–653 McGraw-Hill Inc. NY
Sagawa Y & Kunisaki JT (1984) Clonal propagation: Orchids. In: Cell Culture and Somatic Cell Genetics of Plants I ( Sagawa Y & Kunisaki JT, ed.) pp. 61–67 Academic Press NY
Vacin E & Went F (1949) Some pH changes in nutrient solution. Botan. Gaz. 110: 605–613.
Wimber DE (1963) Clonal multiplication of Cymbidiums through tissue culture of shoot meristem. Amer. Orchid Soc. Bull. 32: 105–107.
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© 1991 Springer Science+Business Media New York
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Sagawa, Y. (1991). Clonal Propagation of Orchids. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0103-2_25
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DOI: https://doi.org/10.1007/978-94-009-0103-2_25
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-011-7658-3
Online ISBN: 978-94-009-0103-2
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