Abstract
The production of transgenic plants has provided new insights into plant biology and has become an important tool for the improvement of crop species (for review, see [2, 15]). Until recently, however, most of the studies using transgenic plants employed dicotyledonous species because of the relative ease of transformation. Monocotyledonous plants are not generally susceptible to Agrobacterium tumefaciens which is routinely used for transformation of dicotyledonous plants. Furthermore, lack of efficient regeneration of fertile plants from protoplasts of monocot species has made it difficult to use the alternative, direct DNA transfer method.
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References
Baba A, Hasazawa S, Shono K (1986) Cultivation of rice protoplasts and their transformation mediated by Agrobacterium spheroplasts. Plant Cell Physiol 27: 463–472
Benfey PN, Chua N-H (1989) Regulated genes in transgenic plants. Science 244: 174–181
Ellis JG, Llewellyn DJ, Dennis ES, Peacock WJ (1987) Maize Adh-1 promoter sequences control anaerobic regulation: addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco. EMBO J 6: 11–16
Harms CT, Potrykus I (1978) Fractionation of polant protoplast types by iso-osmotic density gradient centrifugation. Theor Appl Genet 53: 57–63
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: ßglucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907
Keith B, Chua N-H (1986) Monocot and dicot pre-mRNAs processed with different efficiencies in transgenic tobacco. EMBO J 5: 2419–2425
Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206: 408–413
Kyozuka J, Otoo E, Shimamoto K (1988) Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet 76: 887–890
Kyozuka J, Shimamoto K, Ogura H (1989) Regeneration of plants from rice protoplasts. In Biotechnology in Agriculture and Forestry, Vol. 8, Plant protoplasts and Genetic Engineering I (ed. by Bajaj YSP ) pp. 109–123, Springer-Verlag Berlin, Heidelberg, New York, Tokyo
Kyozuka J, Izawa T, Nakajima M, Shimamoto K (1990) Effect of the promoter and the first intron of maize Adhl on foreign gene expression in rice. Maydica 35: 1–5
Marcotte WR, Christopher CB, Quatrano RS (1988à ) Regulation of a wheat promoter by abscisic acid in rice protoplasts. Nature 335: 454–457
Mundy J, Yamaguchi-Shinozaki K, Chua NH (1990) Nuclear proteins bind conserved elements in the abscisic acid-responsive promoter of rice rab gene. Proc Natl Acad Sci USA 87: 1406–1410
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Ohira K, Ojima K, Fujiwara A (1973) Studies on the nutrition of rice cell culture 1. A simple, defined medium for rapid growth in suspension culture. Plant Cell Physiol 14: 1113–1121
Schell JS (1987) Transgenic plants as tools to study the molecular organization of plant genes. Science 237: 1176–1182
Shimamoto K, Terada R, Izawa T, Fujimoto H (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338: 274–276
Terada R, Shimamoto K (1990) Expression of CaMV35 S-GUS gene in transgenic rice plants. Mol Gen Genet 220: 389–392
Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Transgenic rice plants after direct gene transfer into protoplasts. Bio/Technology 6: 1072–1074
Uchimiya H, Fushimi T, Hashimoto H, Harada H, Shono K, Sugawara Y (1986) Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.) Mol Gen Genet 204: 204–207
Wesley BB, Christensen AH, Klein T, Fromm M, Quail PH (1989) Photoregulation of a phytochrome gene promoter from oat transformed into rice by particle bombardment. Proc Natl Acad Sci USA 86: 9692–9696
Zhang W, Wu R (1988) Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in plants. Theor Appl genet 76: 835–840
Zhang HM, Yang H, Reach EL, Golds TJ, Davis AS, Mulligan BJ, Cocking EC, Davey MR (1988) Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts. Plant Cell Rep 7: 379–384
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© 1991 Springer Science+Business Media New York
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Kyozuka, J., Shimamoto, K. (1991). Transformation and regeneration of rice protoplasts. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0103-2_13
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DOI: https://doi.org/10.1007/978-94-009-0103-2_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-011-7658-3
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