Abstract
In order to elucidate the function of axil, which is directing auxin independent growth in tobacco (Nicotiana tabacum) protoplasts, the expression pattern of the gene was examined under various conditions. It has been shown previously that axi1 is expressed mainly in root and apical meristem in cells which are frequently dividing. We wish to know how plant cell division is controlled in relation with the expression of axi1 gene. Since wounding induces dedifferentiation and cell division, the effect of wounding on axi1 expression was investigated by the method of reverse transcription-polymerase chain reaction (RT-PCR). Data we presented which show accumulation of axi1 mRNA stimulated by mechanical wounding not only on the wounded leaves but also on the adjacent unwounded leaves.
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References
Hayashi, H, I Czaja, H Lubenow, J Schell and R Walden 1992 Science 258 (20), 1350–1353.
Hemerly, A S, P Ferreira, J D A Engler, Montagu M Van, G Engler, and D Inze 1993 Plant Cell 5, 1711–1723.
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© 1997 Kluwer Academic Publishers
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Mano, H., Fujimaki, S., Hayashi, H., Chino, M. (1997). Wounding inducibility of axil gene, which induces auxin independent cell division of tobacco protoplast. In: Ando, T., Fujita, K., Mae, T., Matsumoto, H., Mori, S., Sekiya, J. (eds) Plant Nutrition for Sustainable Food Production and Environment. Developments in Plant and Soil Sciences, vol 78. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0047-9_55
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DOI: https://doi.org/10.1007/978-94-009-0047-9_55
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6510-8
Online ISBN: 978-94-009-0047-9
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