Abstract
Uricase II (nodulin-35) is believed to regulate allantoin biosynthesis in nodules of various legumes. The mechanisms of nodule-specific enhancement of nodulin-35 gene expression were studied in soybeans (Glycine max. L. cv. akisengoku). Northern blot hybridization assay detected only very low levels of nodulin-35 gene expression in various non-symbiotic tissues of soybean plants; only in nodule tissues was the expression enhanced significantly. RT-PCR assay showing enhanced nodulin-35 expression was observed in only one (UR9) of two nodulin-35 genes (UR2 and UR9) in the soybean genome.
A reporter gene expression analysis using the transgenic hairy root system suggested that the cis-element regulating uninfected cell-specific expression in nodules is located between the -200 to -400 bp region of the UR9 gene.
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Tajima S, Takane K, Ohkawa K, Sugimoto A and Okazaki K 1996 Plant Cell Physiol. 37, 1188–1192.
Takane K, Tanaka K, Kouchi H, Okazaki H and Tajima S 1997 Plant Cell Physiol. 38, 149–154.
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© 1997 Kluwer Academic Publishers
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Tajima, S., Takane, K., Kouchi, H. (1997). Uninfected cell specific enhancement of a nodulin-35 genomic gene expression in soybean nodules. Evidences by RT-PCR and chimeric promoter region: GUS reporter gene analysis using transgenic hairy root system of soybean. In: Ando, T., Fujita, K., Mae, T., Matsumoto, H., Mori, S., Sekiya, J. (eds) Plant Nutrition for Sustainable Food Production and Environment. Developments in Plant and Soil Sciences, vol 78. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0047-9_49
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DOI: https://doi.org/10.1007/978-94-009-0047-9_49
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6510-8
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