Abstract
Detection and identification of phytoplasmas (mycoplasma like organisms, MLOs) by conventional techniques such as DAPI staining followed by fluorescence microscopy, ELISA or biological indexing is limited by relatively low sensitivity and unspecificity of observed symptoms (Seemüller, 1989). Recently, rapid and sensitive methods of phytoplasma detection, based on the enzymatic amplification of pathogen DNA, were developed (Deng and Hiruki, 1990; Ahrens and Seemüller, 1992; Lee et al., 1993; Kirkpatrick et al., 1994). These methods were used successfully in our study for the diagnosis of two diseases observed in Poland.
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Literature
Ahrens U., Seemtiller E., 1992. Detection of DNA of Plant Pathogenic Mycoplasmalike Organisms by aPolymerase Chain Reaction that Amplifies a Sequence of the 16S rRNA Gene. Phytopathology 82: 828–832.
Deng S. and Hiruki C., 1990. Enhanced detection of a plant pathogenic mycoplasma-like organism by using polymerase chain reaction. Proc. Japan Acad. 66: 140–144.
Kirkpatrick B., Smart C., Blomquist C., Guerra L., Harrison N., Ahrens U., Lorenz K.H., Scheider B., Seemüller E., 1994. Identification of MLO strain-specific primers obtained from 16/23S spacer sequences. IOM Letters 3: 261–262.
Lorenz K.H., Schneider B., Ahrens U., Seemüller E., 1995. Detection of the Apple proliferation and Pear decline phytoplasmas by PCR Amplification of Ribosomal and Nonribosomal DNA. Phytopathology 85: 771–776.
Lee I.M., Hammond R.W., Davis R.E., Gundersen D.E., 1993. Universal Amplification and Analysis of Pathogen 16S rDNA for Classification and Identification of Mycoplasmalike Organisms. Phytopathology 83: 834–842.
Saiki R.K., Gelfand D.H., Stoffel S., Scharf S.J., Higuchi R., Horn G.T., Mullis K.T., 1988. Primer- Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase. Science 239: 487–491.
Schneider B., Ahrens U., Kirkpatrick B.C., Seemüller E., 1993. Classification of plant-pathogenic mycoplasma-like organisms using restriction-site analysis of PCR-amplified 16S rDNA. J.Gen.Microbiol. 139: 519–527.
Seemüller E., 1989. Pear Decline. In: Virus and viruslike diseases of pome fruits and simulating noninfectious disorders. Ed. P.F. Fridlund, pp. 188–201. Washington State University, Cooperative Extension, Special Publication No. SP0003.
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© 1997 Kluwer Academic Publishers
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Malinowski, T., Komorowska, B., Kamińska, M., Zawadzka, B. (1997). Application of Polymerase Chain Reaction (PCR) for the Positive Identification of Two Phytoplasma Diseases in Poland. In: Dehne, HW., Adam, G., Diekmann, M., Frahm, J., Mauler-Machnik, A., van Halteren, P. (eds) Diagnosis and Identification of Plant Pathogens. Developments in Plant Pathology, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0043-1_96
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DOI: https://doi.org/10.1007/978-94-009-0043-1_96
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