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The Use of Nested PCR for the Detection of Phytophthora Fragariae in Strawberry

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Diagnosis and Identification of Plant Pathogens

Part of the book series: Developments in Plant Pathology ((DIPP,volume 11))

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Abstract

The fungus Phytophthora fragariae Hickman is a quarantine organism and is as well pathogenic on strawberry as on raspberry [1]. It means that all plantmaterial for export has to be tested on the presence of the fungus. A sensitive and specific detection method is therefore necessary. At IPO-DLO in the Department of Detection we work on the development of such a detection method. In the past work was done to solve this problem by serological methods [2,3,4,5]. Unfortunately with limited succes. In this study a detection method is developed using nested PCR.

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Author information

Authors and Affiliations

  1. Institute for Plant Protection (IPO-DLO), PO BOX 9060, 6700, Wageningen, GW, The Netherlands

    P. J. M. Bonants, M. Hagenaar-De Weerdt & M. P. E. van Gentpelzer

  2. Scottish Crop Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, UK

    I. Lacourt, D. E. L. Cooke & J. M. Duncan

Authors
  1. P. J. M. Bonants
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  2. M. Hagenaar-De Weerdt
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  3. M. P. E. van Gentpelzer
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  4. I. Lacourt
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  5. D. E. L. Cooke
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  6. J. M. Duncan
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Editor information

Editors and Affiliations

  1. Institute for Plant Diseases, Rheinische Friedrich-Wilhelms-Universitaet Bonn, Nussallee 9, D-53115, Bonn, Germany

    H.-W. Dehne

  2. Institute of Applied Botany, Dept. of Plant Protection, University of Hamburg, Germany

    G. Adam

  3. International Plant Genetic Resources Institute, Via delle Sette Chiese 142, 00145, Rome, Italy

    M. Diekmann

  4. Landwirtschaftskammer Westfalen-Lippe, Institut für Pflanzenschutz, Nevinghoff 40, 48147, Münster, Germany

    J. Frahm

  5. Agrochemicals Division, Research / Fungicides, Bayer AG, D-51368, Leverkusen, Germany

    A. Mauler-Machnik

  6. Plant Protection Service, P.O. Box 80048, 6700 HC, Wageningen, The Netherlands

    P. van Halteren

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© 1997 Kluwer Academic Publishers

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Bonants, P.J.M., Weerdt, M.HD., van Gentpelzer, M.P.E., Lacourt, I., Cooke, D.E.L., Duncan, J.M. (1997). The Use of Nested PCR for the Detection of Phytophthora Fragariae in Strawberry. In: Dehne, HW., Adam, G., Diekmann, M., Frahm, J., Mauler-Machnik, A., van Halteren, P. (eds) Diagnosis and Identification of Plant Pathogens. Developments in Plant Pathology, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0043-1_9

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  • DOI: https://doi.org/10.1007/978-94-009-0043-1_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6508-5

  • Online ISBN: 978-94-009-0043-1

  • eBook Packages: Springer Book Archive

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