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Silver-Ion Chromatography of Glycerolipids

  • Living reference work entry
  • First Online:
Encyclopedia of Lipidomics

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Synonyms

Ag-LC of glycerolipids

Introduction

The ability of silver ion and some other metal ions to interact with double bonds (DBs) is well known for a long time (Dewar 1951). Silver ion chromatography, sometimes called argentation chromatography, is a separation technique based on the formation of weak reversible charge-transfer complexes between silver ion and DB of unsaturated organic molecules. Silver ion chromatography can be performed either in a planar arrangement (silver ion thin layer chromatography) or in column, which is often used for preparative purposes or silver ion high-performance liquid chromatography (Ag-HPLC) used for high-resolution separations.

The retention in the Ag-HPLC is governed by the DB number. More DB means stronger interactions and therefore higher retention times. In practice, the situation is not as simple because other factors have to be taken into account, such as the DB geometry (cis vs. trans), distances among individual DB (from the conjugation...

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Authors and Affiliations

  1. Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentská 573, 53210, Pardubice, Czech Republic

    Michal Holčapek & Miroslav Lísa

Authors
  1. Michal Holčapek
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  2. Miroslav Lísa
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Correspondence to Michal Holčapek .

Editor information

Editors and Affiliations

  1. Yong Loo Lin School of Medicine Centre for Life Sciences, Singapore, Singapore

    Markus R. Wenk

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Holčapek, M., Lísa, M. (2016). Silver-Ion Chromatography of Glycerolipids. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_74-1

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  • DOI: https://doi.org/10.1007/978-94-007-7864-1_74-1

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