Abstract
Plant-based environmental remediation has been widely pursued by academic and industrial scientists as a favorable low-cost clean-up technology. Phytoremediation is being developed as an alternative technology for removing or, more accurately, reducing the concentration of toxic pollutants to clean up the environment. In the present research, potential of green plants have been screened for phytoremediation of heavy metals both from aquatic and terrestrial environment. Indian mustard (Brassica juncea) has been found as a potential candidate for phytoremediation of heavy metals. B. juncea has been used for remediation of Cd, Pb and Zn at varying concentrations, viz., 0, 5, 10, 20 and 50 ppm. The depletion of heavy metals was observed at the intervals of 0, 1, 3, 7, 14 and 21 days and metal uptake was studied in the roots/shoots of the plants. The percentage removal of Cd, Pb and Zn was found 88.9%, 80% and 89.8%, respectively at the higher exposure concentration (50 ppm). Similarly B. juncea has also been used for phytoremediation of heavy metals (Cd, Pb and Zn) at varying concentrations, viz., 0, 5, 10, 20 and 50 mg/kg from mycorrhizal soil in pot culture technique and uptake was studied in the roots/shoots; after harvesting the plants. The uptake of metals in roots was found 25,000 μg g−1 – Cd, 32,750 μg g−1 -Pb and 30,550 μg g−1 –Zn; whereas uptake in shoots was found 4,596 μg g−1 Cd, 3,469 μg g−1 Pb and 15,878 μg g−1 Zn at higher exposure concentration (50 ppm). The research study has proved effective remediation of heavy metals (Cd, Pb and Zn) by B. juncea in water-soil environment.
Keywords
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Abbreviations
- Cd:
-
Cadmium
- cm:
-
Centimeter
- kg:
-
Kilogram
- μg:
-
Micro gram
- μl:
-
Micro liter
- mg:
-
Milli gram
- ml:
-
Milli liter
- MS:
-
Mycorrhizal soil
- Pb:
-
Lead
- ppm:
-
Parts per million
- VAM:
-
Vesicular arbuscular mycorrhiza
- Zn:
-
Zinc
References
Aboulroos SA, Helal MID, Kamel MM (2006) Remediation of Pb and Cd polluted soils using in situ immobilization and phytoextraction techniques. Soil Sediment Contam 15:199–215
Anamika S, Fulekar MH (2010) Impact of heavy metals in mycorrhizosphere: strategy for phytoremediation. Can J Pure Appl Sci 4:1293–1302
Anamika S, Eapen S, Fulekar MH (2009) Potential of Medicago sativa for uptake of cadmium from contaminated environment. Roman Biotechnol Lett 14:4164–4169
Angle JS, Linacre NA (2005) Metal phytoextraction – a survey of potential risks. Int J Phytoremediation 7:241–254
APHA, AWWA, WEF (1998) Standard methods for the examination of water and wastewater. The Association, Washington, DC
Augé RM, Stodola AJW, Tims JE, Saxton AM (2001) Moisture retention properties of a mycorrhizal soil. Plant Soil 230:87–97
Bearden BN, Petersen L (2000) Influence of arbuscular mycorrhizal fungi on soil structure and aggregate stability of a vertisol. Plant Soil 218:173–183
Bennett LE, Burkhead JL, Hale KL, Terry N, Pilon M, Pilon-smits EAH (2003) Analysis of transgenic Indian mustard plants for phytoremediation of metals-contaminated mine tailings. J Environ Qual 32:432–440
BIO-WISE (2000) Contaminated land remediation: a review of biological technology. DTI, London
Bush JK (2008) The potential role of mycorrhizae in the growth and establishment of Juniperus seedlings. In: Van Auken OW (ed) Western north American Juniperus communities. Springer, New York, pp 111–130
Chaney RL, Malik M, Li YM, Brown SL, Brewer EP, Angel JS, Baker AJM (1997) Phytoremediation of soil metals. Curr Opin Biotechnol 8:279–284
Chowdhury BA, Chandra RK (1987) Biological and health implications of toxic heavy metals and essential trace element interactions. Prog Food Nutr Sci 11:55–113
Cui Y, Wang Q, Christie P (2004) Effect of elemental sulphur on uptake of cadmium, zinc and sulphur by oilseed rape growing in soil contaminated with zinc and cadmium. Commun Soil Sci Plant Anal 35:2905–2916
Cunningham SD, Berti WR, Huang JW (1995) Phytoremediation of contaminated soils. Trends Biotechnol 13:393–397
Flathman PE, Lanza GR (1998) Phytoremediation: current views on an emerging green technology. J Soil Contam 7:415–432
Fulekar MH (2005a) Bioremediation technologies for environment. India J Environ Prot 25:358–364
Fulekar MH (2005b) Environmental biotechnology. Oxford & IBH, New Delhi
Gaur A, Adholeya A (2004) Prospects of arbuscular mycorrhizal fungi in phytoremediation of heavy metal contaminated soils. Curr Sci 86:528–534
Gisbert C, Ros R, De Haro A, Walker DJ, Pilar BM, Serrano R, Avino JN (2003) A plant genetically modified that accumulates Pb is especially promising for phytoremediation. Biochem Biophys Res Commun 303:440–445
Greger M (2004) Metal availability, uptake, transport and accumulation in plants. In: Prasad MN (ed) Heavy metal stress in plants: from biomolecules to ecosystems, 2nd edn. Springer, Berlin
Hoagland DR, Arnon DI (1938) The water culture method for growing plants without soil. Calif Agric Exp Stn Circ 3:346–347
Igwe JC, Abia AA (2006) A bio-separation process for removing heavy metals from waste water using biosorbents. Afr J Biotechnol 5:1167–1179
Jackson ML (1973) Soil chemical analysis. Prentice Hall of India, New Delhi
Jadia CD, Fulekar MH (2008) Phytotoxicity and remediation of heavy metals by fibrous root grass in soil – vermicompost media. J Appl Biosci 10:491–499
Jing Y, He Z, Yang X (2007) Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils. J Zhejiang Univ Sci B 8:192–207
Kim IS, Kang KH, Green PJ, Lee EJ (2003) Investigation of heavy metal accumulation in Polygonum thunbergii for phytoextraction. Environ Pollut 126:235–243
Kumar PBAN, Dushenkov V, Motto H, Raskin I (1995) Phytoextraction: the use of plants to remove heavy metals from soils. Environ Sci Technol 29:1232–1238
Lai H-Y, Chen Z-S (2009) In-situ selection of suitable plants for the phytoremediation of multi-metals-contaminated sites in central Taiwan. Int J Phytoremediation 11:235–250
Lane SD, Martin ES (1977) A histochemical investigation of lead uptake in Raphanus sativus. New Phytol 79:281–286
Lasat MM (2002) Phytoremediation of toxic metals: a review of biological mechanisms. J Environ Qual 31:109–120
Lewandowski I, Schmidt U, Londo M, Faaij A (2006) The economic value of the phytoremediation function- assessed by the example of cadmium remediation by willows (Salix sp.). Agric Syst 89:68–89
Leyval C, Turnau K, Haselwandter K (1997) Effect of heavy metal pollution on mycorrhizal colonization and function: physiological, ecological and applied aspects. Mycorrhiza 7:139–153
Liu JR, Suh M, Ch Choi D (2000) Phytoremediation of cadmium contamination: overexpression of metallothionein in transgenic tobacco plants. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 43:126–130
Lone MI, He Z, Stoffella PJ, Yang X (2008) Phytoremediation of heavy metal polluted soils and water: progress and perspectives. J Zhejiang Univ Sci B 9:210–220
Mahajan BK (1997) Methods in biostatistics for medical students and research workers, 6th edn. Jaypee Brothers, New Delhi
McGrath SP, Lombi E, Gray CW, Caille N, Dunham SJ, Zhao FJ (2006) Field evaluation of Cd and Zn phytoextraction potential by the hyperaccumulators Thlaspi caerulescens and Arabdopsis halleri. Environ Pollut 141:115–125
Meagher RB (2000) Phytoremediation of toxic elemental and organic pollutants. Curr Opin Plant Biol 3:153–162
Miller RM, Jastrow JD (1990) Hierarchy of root and mycorrhizal fungal interactions with soil aggregation. Soil Biol Biochem 22:579–584
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Okoronkwo NE, Igwe JC, Onwuchekwa EC (2005) Risk and health implication of polluted soils for crop production. Afr J Biotechnol 4:1521–1524
Pivetz BE (2001) Phytoremediation of contaminated soil and ground water at hazardous waste sites. EPA ORD Ground Water Issue, EPA/540/S–01/500
Salt DE, Blaylock M, Kumar PBAN, Dushenkov V, Ensley BD, Chet I, Raskin I (1995) Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants. Biotechnol 13:468–475
Salt DE, Smith RD, Raskin I (1998) Phytoremediation. Annu Rev Plant Physiol Plant Mol Biol 49:643–668
Schneider T, Haag-Kerwer A, Maetz M, Niecke M, Povh B, Rausch T (1999) Micro-PIXE studies of elemental distribution in Cd-accumulating Brassica juncea L. Nucl Instrum Methods Phys Res B: Interact Mater Atoms 158:329–334
Schnoor JL (2002) Technology evaluation report: phytoremediation of soil and groundwater. GWRTAC Series TE-02-01
USEPA (1997) Cleaning Up the Nation’s waste sites: markets and technology trends, united states environmental protection agency EPA/542/R-96/005. Office of Solid Waste and Emergency Response, Washington, DC
Vázquez MD, Barceló J, Poschenrieder C, Mádico J, Hatton P, Baker AJM, Cope GH (1992) Localization of zinc and cadmium in Thlaspi caerulescens (Brassicaceae), a metallophyte that can hyperaccumulate both metals. J Plant Physiol 140:350–355
Weber O, Scholz RW, Bühlmann R, Grasmück D (2001) Risk perception of heavy metals soil contamination and attitudes toward decontamination strategies. Risk Anal 21:967–977
Wei S, Jaime A, Da Silva T, Zhou Q (2008) Agro-improving method of phytoextracting heavy metal contaminated environment. J Hazard Mater 150:662–668
Ximenez-Embun P, Madrid-Albarran Y, Camara C, Cuadrado C, Burbano C, Muzquiz M (2001) Evaluation of lupines species to accumulate heavy metals from waste waters. Int J Phytoremediation 3:369–379
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Singh, A., Fulekar, M.H. (2012). Phytoremediation of Heavy Metals by Brassica juncea in Aquatic and Terrestrial Environment. In: Anjum, N., Ahmad, I., Pereira, M., Duarte, A., Umar, S., Khan, N. (eds) The Plant Family Brassicaceae. Environmental Pollution, vol 21. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-3913-0_6
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