Abstract
Since their functional role in tumour biology is to a large extent unknown, the focus of the current study is the extraction and quantification of circulating microRNAs. Using serum of patients with ovarian cancer, the suitability of these molecules as new blood-based markers for prognosis is examined. The concentrations of four circulating microRNAs (miRNA10b, miRNA34a, miRNA141 and miRNA155) were measured in serum of 59 patients with ovarian cancer and 29 healthy individuals by a TaqMan MicroRNA Assay. Serum miRNA levels significantly discriminated tumour patients from healthy individuals. In blood of ovarian cancer patients the levels of total RNA, miRNA10b, miRNA34a, miRNA141, and miRNA155, were significantly higher than those from healthy controls. Repression of miRNA141 and miRNA155 seems to be regulated by DNA methylation. Statistical correlations showed a significant correlation of increasing miRNA34a amounts with lymph node metastases.
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We thank Bettina Steinbach for her excellent technical assistance.
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Roth, C., Kasimir-Bauer, S., Heubner, M., Pantel, K., Schwarzenbach, H. (2010). Increase in Circulating MicroRNA Levels in Blood of Ovarian Cancer Patients. In: Gahan, P. (eds) Circulating Nucleic Acids in Plasma and Serum. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9382-0_9
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DOI: https://doi.org/10.1007/978-90-481-9382-0_9
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