Abstract
With the aim to facilitate high-throughput genome profiling and genetic and physical mapping within the Festuca-Lolium complex, we have developed a Diversity Arrays Technology (DArT) array for five important species: Festuca pratensis, Festuca arundinacea, Festuca glaucescens, Lolium perenne and Lolium multiflorum. The DArTFest array contains 7,680 probes derived from methyl-filtered genomic representations. Of 3,884 polymorphic DArT markers identified in the first marker discovery experiment, over 1,000 markers detected a positive allele in each species.
We assigned DArT markers to individual chromosome regions of F. pratensis using a series of single chromosome substitution and recombinant lines of F. pratensis in L. multiflorum. Moreover, we enriched the existing genetic map of F. pratensis by over 200 DArT markers and existing genetic map of L. multiflorum by over 500 DArT markers. The resources developed in this project will facilitate development of genetic maps in Festuca and Lolium, the analysis of genomic constitution in Festuca × Lolium hybrids, as well as marker-assisted selection for multiple traits.
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Acknowledgements
This work was supported by the Ministry of Agriculture of the Czech Republic (grant award NAZV QH71267) and the Czech Science Foundation (grant award 521/07/P479).
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Kopecký, D. et al. (2010). DArTFest – A Platform for High-Throughput Genome Profiling Within the Festuca – Lolium Complex. In: Huyghe, C. (eds) Sustainable use of Genetic Diversity in Forage and Turf Breeding. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8706-5_65
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DOI: https://doi.org/10.1007/978-90-481-8706-5_65
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