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Concentration and Purification of Densonucleosis Virus by Tangential Membrane Filtration and by Ion Exchange Membranes

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Cells and Culture

Part of the book series: ESACT Proceedings ((ESACT,volume 4))

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Abstract

In gene therapy and vaccine production, large-scale purification of virus vectors is often essential. In the manufacture of biopharmaceuticals, validation of virus clearance is critical. Tangential flow filtration is well established in the biotechnology industry for the purification and concentration of proteins. Also chromatography is widely used in downstream processing and the replacement of resins by membranes as chromatographic supports is of interest to overcome limitations associated with resin-based chromatography. Purification of the Aedes aegypti densonucleosis virus (AeDNV) using tangential flow filtration and also using anion and cation exchange membranes was investigated. The results showed that 30, 50, and 100 kDa membranes reject the virus particles in contrast to 300 kDa membranes. The permeate flux is strongly affected by the used medium. AeDNV particles may be adsorbed most effective by strongly basic anion exchange membranes.

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Author information

Authors and Affiliations

  1. Institute of Biopharmaceutical Technology, University of Applied Sciences, Wiesenstrasse 14, 35390, Giessen, Germany

    Peter Czermak, David L. Grzenia & Anne Wolf

  2. Department of Chemical Engineering, Kansas State University, Manhattan, KS, USA

    Peter Czermak

  3. Department of Chemical Engineering, Colorado State University, Fort Collins, CO, USA

    David L. Grzenia, Rachel Specht, Binbing Han & S. Ranil Wickramasinghe

  4. Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA

    Jonathan O. Carlson

Authors
  1. Peter Czermak
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  2. David L. Grzenia
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  3. Anne Wolf
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  4. Jonathan O. Carlson
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  5. Rachel Specht
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  6. Binbing Han
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  7. S. Ranil Wickramasinghe
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Corresponding author

Correspondence to Peter Czermak .

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Editors and Affiliations

  1. Technische Fak., AG Zellkulturtechnik, Universität Bielefeld, Bielefeld, Germany

    Thomas Noll

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Czermak, P. et al. (2010). Concentration and Purification of Densonucleosis Virus by Tangential Membrane Filtration and by Ion Exchange Membranes. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_90

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