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Release of Sugar Chains from Glycosphingolipids

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Experimental Glycoscience

Abstract

Sugar chains of glycosphingolipids (GSLs) can be released by ozonolysis or periodate oxidation; however, these methods are not suitable for alkaline-sensitive GSLs because they are performed under alkaline conditions. Furthermore, these chemical procedures are time-consuming, somewhat troublesome, and of low yield; and thus a simple and reproducible method for obtaining intact sugar chains from GSLs is required. This section deals with the release of sugar chains from GSLs by endoglycoceramidase (EGCase, EC3.2.1.123).

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References

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Author information

Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Graduate School Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Makoto Ito

Authors
  1. Makoto Ito
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Editor information

Editors and Affiliations

  1. Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, 1-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)

  2. Systems Glycobiology Group, Advanced Science Institute, RIKEN, Wako, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)

  3. Institute of Glycotechnology, Future Science and Technology Joint Research Center, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan

    Akemi Suzuki M.D., Ph.D. (Professor) (Professor)

  4. Synthetic Cellular Chemistry Laboratory, RIKEN Discovery Research Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Yukishige Ito Ph.D. (Chief Researcher) (Chief Researcher)

  5. Research Center for Medical Glycoscience, Advanced Industrial Science and Technology, AIST Tsukuba, Central 2, Tsukuba, Ibaraki, 305-8568, Japan

    Hisashi Narimatsu M.D., Ph.D.

  6. Research Center for Glycobiotechnology, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan

    Toshisuke Kawasaki Ph.D. (Professor) (Professor)

  7. Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan

    Sumihiro Hase Ph.D. (Professor) (Professor)

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© 2008 Springer

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Ito, M. (2008). Release of Sugar Chains from Glycosphingolipids. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77922-3_29

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