Abstract
Most continuous insect cell lines consist of a heterogeneous cell population, probably because the cell lines originate from explants that themselves consist of various tissues or cell types. In addition, it is true that a cell line becomes heterogeneous with repeated passaging. However, it is desirable to have a more homogeneous cell population for use as experimental material. To this end, cell cloning is useful. However, cloned insect cell lines often become heterogeneous again within a short period of time. In this case, recloning or repeated cloning may help to unify the cell population. Cloning is essentially a method of isolating single cells and proliferating the isolated cells. There are several methods by which clones can be obtained.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Itoh, T. and Mitsuhashi, J. (1995) Effects of various conditioned media on proliferation of an isolated single cell from insect cell lines. In Vitro Cell. Dev. Biol. 32A, 814–816. McIntosh, A.H. and Rectoris, C. (1974) Insect cells: colony formation and cloning in agar medium. In Vitro 10, 1–5.
McIntosh, A.H. and Rectoris, C. (1974) Insect cells: colony formation and cloning in agar medium. In Vitro 10, 1–5.
Mitsuhashi, J. (1975) Isolation of a clone from an insect cell line by application of a fibrin clot method. Appl. Entomol. Zool. 10, 309–310.
Peel, D.J. and Milner, M.J (1990) The diversity of cell morphology in cloned cell lines derived from Drosophila imaginal discs. Roux’s Arch. Dev. Biol. 198, 479–482.
Suitor, E.C. Jr.., Chang, L.L. and Liu, H.H (1966) Establishment and characterization of a clone from Grace’s in vitro cultured mosquito (Aedes aegypti L.) cells. Exp. Cell Res. 44, 572–578.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Japan
About this chapter
Cite this chapter
Mitsuhashi, J. (2002). Cell Cloning. In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_31
Download citation
DOI: https://doi.org/10.1007/978-4-431-67875-5_31
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-70313-6
Online ISBN: 978-4-431-67875-5
eBook Packages: Springer Book Archive