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Enhancement by Indole-3-Carbinol of Liver and Thyroid Gland Neoplastic Development in a Rat Medium-Term Multiorgan Carcinogenesis Model

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Food Factors for Cancer Prevention

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Summary

The modifying effects of indole-3-carbinol (I3C), a naturally occurring compound in cruciferous vegetables, were assessed using a rat multiorgan carcinogenesis model. One hundred male Sprague-Dawley (SD) rats were divided randomly into three groups. Groups 1 and 2 were sequentially treated with diethylnitrosamine (DEN), N-methyl-N-nitrosourea (MNU), and dihydroxy-di-N-propylnitrosamine (DHPN) for four weeks (DMD treatment) and Group 3 was given treatment without mutagen. Animals of groups 1 and 3 were given a diet containing 0.25% I3C for 20 weeks after the initiation period, followed by a return to the basal diet for 28 weeks, and subgroups were killed at weeks 24 and 52. I3C caused significant increases in both density and area (mm2/ cm2) of liver cell foci positive for glutathione S-transferase placental form, assessed at week 24 of the experiment (P < 0.01, 0.001). The incidence of hepatocellular adenomas in the DMD → I3C group at week 52 similarly tended to be elevated as compared to the initiation only (DMD-alone) group. The incidence of thyroid gland tumors in the DMD → I3C group was significantly increased compared with the DMD-alone group values at week 52 of the experiment (P < 0.01). In conclusion, I3C was shown to enhance liver and thyroid gland neoplastic development when given during the promotion stage in a rat medium-term multiorgan carcinogenesis model.

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Authors and Affiliations

  1. Chemotherapy Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104, Japan

    Dae Joong Kim & Hiroyuki Tsuda

  2. Department of Pathology, National Institute of Safety Research, Nokbun-dong, Eunpyung-ku, Seoul, 122-020, Korea

    Dae Joong Kim

  3. Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467, Japan

    Nobuyuki Ito

Authors
  1. Dae Joong Kim
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  2. Nobuyuki Ito
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  3. Hiroyuki Tsuda
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Editor information

Editors and Affiliations

  1. Department of Food Science and Technology Faculty of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Kakyo-ku, Kyoto, 606-01, Japan

    Hajime Ohigashi Ph.D. (Professor) (Professor)

  2. Department of Applied Biological Sciences Faculty of Agriculture, Nagoya University, Furocho, Chikusa-ku, Nagoya, Aichi, 464-01, Japan

    Toshihiko Osawa Ph.D. (Professor) (Professor)

  3. Lipid Science Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, 2-1-2 Kannondai, Tsukuba, Ibaraki 305, Japan

    Junji Terao Ph.D. (Head) (Head)

  4. Nutrition and Epidemiology, Department of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156, Japan

    Shaw Watanabe M.D. (Professor) (Professor)

  5. First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602, Japan

    Toshikazu Yoshikawa M.D. (Associate Professor) (Associate Professor)

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© 1997 Springer Japan

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Kim, D.J., Ito, N., Tsuda, H. (1997). Enhancement by Indole-3-Carbinol of Liver and Thyroid Gland Neoplastic Development in a Rat Medium-Term Multiorgan Carcinogenesis Model. In: Ohigashi, H., Osawa, T., Terao, J., Watanabe, S., Yoshikawa, T. (eds) Food Factors for Cancer Prevention. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67017-9_40

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  • DOI: https://doi.org/10.1007/978-4-431-67017-9_40

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-67019-3

  • Online ISBN: 978-4-431-67017-9

  • eBook Packages: Springer Book Archive

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