Abstract
C-type lectins belong to a superfamily of receptors that share structural homology in their carbohydrate recognition domains and often bind to carbohydrates in a Ca2+-dependent fashion. Whereas endocytic C-type lectin receptors (CLRs) trigger the receptor-mediated endocytosis of soluble ligands, myeloid CLRs in innate immunity act as pattern recognition receptors and contribute to the initiation of immune responses. Thus, CLR targeting may serve as a means to mediate cell-specific drug/gene delivery but also to modulate immune responses.
In this chapter, CLR targeting is described using the examples of the asialoglycoprotein receptor (ASGPR) and the DC-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN). Furthermore, a protocol is given that specifies the generation of CLR-Fc fusion proteins. CLR-Fc fusion proteins consist of the extracellular part of the respective CLR and the Fc fragment of IgG molecules. They are helpful tools to identify novel CLR ligands on pathogens or in libraries of synthetic glycan structures. The identified CLR ligands can then be covalently attached to model antigens to analyze their utility for antigen-presenting cell targeting to modulate immune responses.
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References
Cummings RD, McEver RP (2009) C-type Lectins, chapter 31. In: Varki A, Cummings RD, Esko JD, Freeze HH, Stanley P, Bertozzi CR, Hart GW, Etzler ME (eds) Essentials of glycobiology, 2nd edn. Cold Spring Harbor, New York
Lee YC, Lee RT (2000) Interactions of oligosaccharides and glycopeptides with hepatic carbohydrate receptors, chapter 72. In: Ernst B, Hart GW, Sinaý P (eds) Carbohydrates in chemistry and biology. Wiley-WCH, Weinheim
Lepenies B, Yin J, Seeberger PH (2010) Applications of synthetic carbohydrates to chemical biology. Curr Opin Chem Biol 14:404–411
Lepenies B, Lee J, Sonkaria S (2013) Targeting C-type lectin receptors with multivalent carbohydrate ligands. Adv Drug Deliv Rev 65:1271–1281
Maglinao M, Klopfleisch R, Seeberger PH, Lepenies B (2013) The C-type lectin receptor DCIR is crucial for the development of experimental cerebral malaria. J Immunol 191:2551–2559
Maglinao M, Eriksson M, Schlegel MK, Zimmermann S, Johannssen T, Götze S, Seeberger PH, Lepenies B (2014) A platform to screen for C-type lectin receptor-binding carbohydrates and their potential for cell-specific targeting and immune modulation. J Control Release 175C:36–42
Poelstra K, Prakash J, Beljaars L (2012) Drug targeting to the diseased liver. J Control Release 161:188–197
Sancho D, Reis e Sousa C (2012) Signaling by myeloid C-type lectin receptors in immunity and homeostasis. Annu Rev Immunol 30:491–529
Sancho D, Reis e Sousa C (2013) Sensing of cell death by myeloid C-type lectin receptors. Curr Opin Immunol 25:46–52
Singh SK, Streng-Ouwehand I, Litjens M, Weelij DR, GarcÃa-Vallejo JJ, van Vliet SJ, Saeland E, van Kooyk Y (2009) Characterization of murine MGL1 and MGL2 C-type lectins: distinct glycan specificities and tumor binding properties. Mol Immunol 46:1240–1249
van Kooyk Y, Geijtenbeek TB (2003) DC-SIGN: escape mechanism for pathogens. Nat Rev Immunol 3:697–709
van Kooyk Y, Unger WW, Fehres CM, Kalay H, GarcÃa-Vallejo JJ (2013) Glycan-based DC-SIGN targeting vaccines to enhance antigen cross-presentation. Mol Immunol 55:143–145
Zelensky AN, Gready JE (2005) The C-type lectin-like domain superfamily. FEBS J 272:6179–6217
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Johannssen, T., Hütter, J., Zimmermann, S., Lepenies, B. (2015). C-Type Lectins. In: Taniguchi, N., Endo, T., Hart, G., Seeberger, P., Wong, CH. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54841-6_138
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DOI: https://doi.org/10.1007/978-4-431-54841-6_138
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Publisher Name: Springer, Tokyo
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