Abstract
The matrix application procedure is the most critical step of the sample preparation procedure, because it affects co-cystalization of the matrix-analyte complex formed on the tissue surface, thus the quality of the mass spectra and the subsequent imaging results. Several methods have been used for matrix application: (1) manually spraying the matrix solution with a nebulizer sprayer, (2) automatically depositing an array of small droplets of matrix solution with robotic devices, and (3) sublimating the matrices under reduced pressure and elevated temperature. In this chapter, we introduce an overview of these procedures and then continue with details of technical points, especially of methods (1) and (2). Lastly, a Further advanced application method, which is the spray-droplet method to increase signal sensitivity, is also described.
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Sugiura, Y., Setou, M., Horigome, D. (2010). Methods of Matrix Application. In: Setou, M. (eds) Imaging Mass Spectrometry. Springer, Tokyo. https://doi.org/10.1007/978-4-431-09425-8_6
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DOI: https://doi.org/10.1007/978-4-431-09425-8_6
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-09424-1
Online ISBN: 978-4-431-09425-8
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