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Kryokonservierung und Langzeitlagerung von Zellen

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Der Experimentator: Zellkultur

Part of the book series: Experimentator ((EXPERIMENTATOR))

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Zusammenfassung

In einem Zellkulturlabor gibt es immer Zellen oder Zelllinien, die entweder gar nicht mehr oder nur eine Zeit lang, z. B. fur die Dauer eines Projekts, oder nur fur bestimmte experimentelle Zwecke benotigt werden. Diese Zellen auf Sparflamme in der Dauerkultur zu halten ist schon deshalb nicht sinnvoll, weil das Arbeitszeit und Geld kostet, zudem ist Platz im Brutschrank eine kostbare Raritat. Will man das Zellmaterial fur spatere Einsatze aufbewahren, ist die Kryokonservierung eine sinnvolle Masnahme. Ein kleiner Ausflug in die Welt der Kryobiologie schadet daher nicht, sondern hilft , sich fur die Stolpersteine, die ohne Zweifel bei diesem Th ema existieren, zu sensibilisieren.

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Literatur

  • Bank H &. Mazur P (1973) Visualization of freezing damage, J. Cell Biol. 57, 729–742

    Article  PubMed  CAS  Google Scholar 

  • Bank H (1973) Visualization of freezing damage II. Structural alterations during warming, Cryobiology 10, 157–170

    Article  PubMed  CAS  Google Scholar 

  • Diller KR et al. (1972) Intracellular freezing in biomaterials, Cryobiology 9, 429–440

    Article  PubMed  CAS  Google Scholar 

  • Farrant J et al. (1977) Use of two-step cooling procedures to examine factors influencing cell survival following freezing and thawing, Cryobiology 14, 273–286

    Article  PubMed  CAS  Google Scholar 

  • Kryobankbroschüre des Fraunhofer-Instituts für Biomedizinische Technik (download über www.ibmt.fraunhofer.de)

  • Leibo SP & Mazur P (1971) The role of cooling rates in lowtemperature preservation, Cryobiology 8, 447–452

    Article  PubMed  CAS  Google Scholar 

  • Levin RL & Cravalho EG (1976) A membrane model describing the effect of temperature on the water conductivity of erythrocyte membranes at subzero temperatures, Cryobiology 13, 415–429

    Article  PubMed  CAS  Google Scholar 

  • Lovelock JE & Bishop MWH (1959) Prevention of freezing damage to living cells by dimethyl sulfoxide, Nature 183, 1394–1395

    Article  PubMed  CAS  Google Scholar 

  • Lovelock JE (1953) The haemolysis of human red blood-cells by freezing and thawing.

    Google Scholar 

  • Mazur P (1965) Causes of injury in frozen and thawed cells, Fed. Proc. Fed. Amer. Soc Exp Biol 24, 175–182, Suppl. 15

    Google Scholar 

  • Mazur P (1966) Theoretical and experimental effects of cooling and warming velocity on the survival of frozen and thawed cells, Cryobiology, 2, 181–192

    Article  PubMed  CAS  Google Scholar 

  • Mazur P (1970) Cryobiology: The freezing of biological systems, Science 168, 939–949

    Article  PubMed  CAS  Google Scholar 

  • Mazur P et al. (1972) A two-factor hypothesis of freezing injury, Exp. Cell Res 71, 345–355

    Article  CAS  Google Scholar 

  • Meryman HT (1956) Mechanism of freezing in living cells and tissues, Science 124, 515–521

    Article  PubMed  CAS  Google Scholar 

  • Meryman HT (1971) Cryoprotective agents, Cryobiology 8, 173–183

    Article  PubMed  CAS  Google Scholar 

  • Meryman HT (1977) Freezing injury from »solution effects« and its prevention by natural or artificial cryoprotection, Cryobiology 14, 287–302

    Article  PubMed  CAS  Google Scholar 

  • Rall WF & Polge C (1984) Effect of warming rate on mouse embryos frozen and thawed in glycerol, J Reprod Fertil 70, 285–292

    Article  PubMed  CAS  Google Scholar 

  • Smith AU et al. (1951) Microscopic observation of living cells during freezing and thawing., J R Microsc Soc 71(2), 186–195

    Article  PubMed  CAS  Google Scholar 

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© 2011 Spektrum Akademischer Verlag Heidelberg

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Schmitz, S. (2011). Kryokonservierung und Langzeitlagerung von Zellen. In: Der Experimentator: Zellkultur. Experimentator. Spektrum Akademischer Verlag, Heidelberg. https://doi.org/10.1007/978-3-8274-2573-7_12

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