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Detection of Endogenous Albumin as an Index of Blood Parenchymal Border Alteration

  • Conference paper
Brain Edema IX

Part of the book series: Acta Neurochirurgica ((NEUROCHIRURGICA,volume 60))

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Summary

We used a microdialysis technique to establish a method to detect sequential changes in disruption of the blood parenchymal border. Twelve cats were divided into two groups; one group underwent occlusion of the middle cerebral artery for 60 minutes, the other a cold injury model. Microdialysis probes were implanted bilaterally into the white matter, and dialysates were collected successively at 30 minute intervals for 6 hours in the occlusion model and 4 hours in the cold injury model. Regional cerebral blood flow was measured simultaneously using the hydrogen clearance method. The water content of the white matter was measured using specific gravity. The proteins in the dialysate were analyzed using electrophoresis with silver stain, and, with densitometric analysis, the density of the 66.2 kDa band was quantified as albumin. The ratio of this density to the preoperative density was defined as the“albumin index.”On the side of the lesion in the cold injury model, this index significantly increased 3 hours after the cold injury compared with the contralateral side, and a correlation between the water content and this index was observed. The albumin index was believed to indicate the severity of disruption of the blood parenchymal border.

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Author information

Authors and Affiliations

  1. Okayama University Medical School, Okayama, Japan

    M. Gotoh, M. Kawauchi, S. Asari & T. Ohmoto

  2. Departments of Neurological Surgery and Neurology, Okayama University Medical School, Okayama, 700, Japan

    Tom Fukuhara M.D., K. Tsutsui & T. Shohmori

Authors
  1. Tom Fukuhara M.D.
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  2. M. Gotoh
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  3. M. Kawauchi
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  4. S. Asari
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  5. T. Ohmoto
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  6. K. Tsutsui
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  7. T. Shohmori
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Editor information

Editors and Affiliations

  1. Department of Neurosurgery, Musashino Red-Cross Hospital, Tokyo, Japan

    Umeo Ito M.D. (Director and Neurosurgeon in Chief) (Director and Neurosurgeon in Chief)

  2. Institut für Chirurgische Forschung, Ludwig-Maximilians-Universität, München, Federal Republic of Germany

    Alexander Baethmann M.D. (Professor) (Professor)

  3. Max-Planck Institut für Neurologische Forschung, Köln, Federal Republic of Germany

    Konstantin-A. Hossmann M.D. (Professor) (Professor)

  4. Department of Neuropathology, Tokyo Medical and Dental University, Tokyo, Japan

    Toshihiko Kuroiwa M.D. (Associate Professor) (Associate Professor)

  5. Division of Neurosurgery, Medical College of Virginia, Richmond, Virginia, USA

    Anthony Marmarou Ph.D. (Professor) (Professor)

  6. Neurochirurgische Klinik, Ludwig-Maximilians-Universität, München, Federal Republic of Germany

    Hans-J. Reulen M.D. (Professor) (Professor)

  7. Department of Neurosurgery, Tokyo Women’s Medical College, Tokyo, Japan

    Kintomo Takakura M.D. (Professor) (Professor)

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© 1994 Springer-Verlag

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Fukuhara, T. et al. (1994). Detection of Endogenous Albumin as an Index of Blood Parenchymal Border Alteration. In: Ito, U., et al. Brain Edema IX. Acta Neurochirurgica, vol 60. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9334-1_32

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  • DOI: https://doi.org/10.1007/978-3-7091-9334-1_32

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-9336-5

  • Online ISBN: 978-3-7091-9334-1

  • eBook Packages: Springer Book Archive

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