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In vivo transfection by hepatitis A virus synthetic RNA

  • Conference paper
Positive-Strand RNA Viruses

Part of the book series: Archives of Virology Supplementum ((ARCHIVES SUPPL,volume 9))

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Summary

Marmosets injected intrahepatically with nucleic acids (cDNA and RNA transcripts) representing the full-length genome of the wild-type HM-175 strain of hepatitis A virus experienced acute hepatitis and seroconversion to hepatitis A virus capsid proteins. The hepatitis was comparable in severity to that caused by infection with the wild-type virus. The viral cDNA and the hepatitis A virus recovered from the feces of an injected animal contained the same marker mutation. Therefore, intermediate cell culture steps can be omitted and the virulence of a hepatitis A virus encoded by a cDNA clone can be evaluated by direct transfection of marmosets.

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References

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Author information

Authors and Affiliations

  1. National Institutes of Health, Bethesda, Maryland, USA

    Dr. S. U. Emerson, M. Lewis & R. H. Purcell

  2. Rancho Los Amigos Medical Center, Downey, California, USA

    S. Govindarajan

  3. Bioqual, Inc., Rockville, Maryland, USA

    M. Shapiro & T. Moskal

  4. Hepatitis Viruses Section, Building 7, Room 203, 9000 Rockville Pike, Bethesda, MD, 20892, USA

    Dr. S. U. Emerson

Authors
  1. Dr. S. U. Emerson
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  2. M. Lewis
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  3. S. Govindarajan
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  4. M. Shapiro
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  5. T. Moskal
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  6. R. H. Purcell
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Editor information

Editors and Affiliations

  1. Department of Biology, Georgia State University, Atlanta, Georgia, USA

    Margo A. Brinton

  2. Arthropod-borne and Infectious Disease Laboratory (AIDL), Colorado State University, Fort Collins, Colorado, USA

    Charles H. Calisher

  3. Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin, USA

    Roland Rueckert

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© 1994 Springer-Verlag

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Emerson, S.U., Lewis, M., Govindarajan, S., Shapiro, M., Moskal, T., Purcell, R.H. (1994). In vivo transfection by hepatitis A virus synthetic RNA. In: Brinton, M.A., Calisher, C.H., Rueckert, R. (eds) Positive-Strand RNA Viruses. Archives of Virology Supplementum, vol 9. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9326-6_20

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  • DOI: https://doi.org/10.1007/978-3-7091-9326-6_20

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82522-8

  • Online ISBN: 978-3-7091-9326-6

  • eBook Packages: Springer Book Archive

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