Summary
It is accepted that the ionic composition of the medium perfused through a microdialysis probe should match that of the extracellular fluid (ECF) under physiological conditions. In contrast, the possibility that control artificial cerebrospinal fluid may influence the experimental or pathological conditions under study, by buffering changes in the ECF composition, has been neglected. Spreading depression (SD) is a propagating transient suppression of electrical activity due to cellular depolarization which may contribute to neuronal damage in focal ischaemia, and underlie the migraine aura. Here we report that microdialysis markedly inhibits SD propagation, by buffering the sudden increase in extracellular K+ associated with this event. This effect is independent of the microdialysis flow rate and does not result from tissue injury following probe implantation. This finding clearly illustrates that microdialysis can influence the pathological conditions under investigation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Benveniste H, Hüttemeier PC (1990) Microdialysis-theory and application. Prog Neurobiol 35: 195–215
De-Boer P, Damsma G, Fibiger HC, Timmerman W, De Vries JB, Westerink BHC (1990) Dopaminergic-cholinergic interactions in the striatum: the critical significance of calcium concentrations in brain microdialysis. Naunyn Schmiedebergs Arch Pharmacol 342: 528–534
Fabricius M, Jensen LH, Lauritzen M (1993) Microdialysis of interstitial amino acids during spreading depression and anoxic depolarization in rat neocortex. Brain Res 612: 61–69
Hansen AJ, Zeuthen T (1981) Extracellular ion concentrations during spreading depression and ischemia in rat brain cortex. Acta Physiol Scand 113: 437–445
Kraig RP, Nicholson C (1978) Extracellular ionic variations during spreading depression. Neuroscience 3: 1045–1059
Obrenovitch TP, Richards DA, Sarna GS, Symon L (1993) Combined intracerebral microdialysis and electrophysiological recording: methodology and applications. J Neurosci Methods 47: 139–145
Obrenovitch TP, Urenjak J, Zilkha E (1994) Intracerebral microdialysis combined with recording of extracellular field potential: a novel method for investigation of depolarizing drugs in vivo. Br J Pharmacol 113: 1295–1302
Obrenovitch TP, Zilkha E (1995) High extracellular potassium, and not extracellular glutamate, is required for the propagation of spreading depression. J Neurophysiol 3: 2107–2114
Rutecki PA, Lebeda FJ, Johnston D (1985) Epileptiform activity induced by changes in extracellular potassium in hippocampus. J Neurophysiol 5: 1363–1373
Scheller D, Heister U, Kolb J, Tegtmeier F (1993) On the role of excitatory amino acids during generation and propagation of spreading depressions. In: Lemenkühler A et al (eds) Migraine: basic mechanisms and treatments. Urban and Schwarzenberg, Munich, pp 355–366
Spira ME, Yarom Y, Zeldes D (1984) Neuronal interactions mediated by neurally evoked changes in the extracellular potassium concentration. J Exp Biol 112: 179–197
Wahl F, Obrenovitch TP, Hardy AM, Plotkine M, Boulu R, Symon L (1994) Extracellular glutamate during focal cerebral ischaemia in rats: time course and calcium dependency. J Neurochem 63: 1003–1011
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag/Wien
About this paper
Cite this paper
Obrenovitch, T.P., Zilkha, E. (1996). Intracerebral Microdialysis Markedly Inhibits the Propagation of Cortical Spreading Depression. In: Mendelowitsch, A., Langemann, H., Alessandri, B., Landolt, H., Gratzl, O. (eds) Clinical Aspects of Microdialysis. Acta Neurochirurgica Supplement, vol 67. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6894-3_4
Download citation
DOI: https://doi.org/10.1007/978-3-7091-6894-3_4
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-7426-5
Online ISBN: 978-3-7091-6894-3
eBook Packages: Springer Book Archive