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Comparison of Bacteriorhodopsin Conformational Changes with Uniaxially Elongated Aliphatic Polyamide Samples by Using FT-IR Difference Spectroscopy

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Progress in Fourier Transform Spectroscopy

Part of the book series: Mikrochimica Acta Supplement ((MIKROCHIMICA,volume 14))

  • 43 Accesses

Abstract

FT-IR difference spectra for the photoreactions of bacteriorhodopsin (bR) are compared to difference spectra of polyamide-6 and -11 obtained during uniaxial elongation. The purple membrane was hydrated with D2O in order to identify bands above 3000 cm−1. In the bR photocycle (bR → K), the difference spectra in D2O closely match the band contour found in the difference spectra of elongated polyamide-11. The data suggest that a structural change occurs in the backbone of the membrane protein bacteriorhodopsin, resulting in weakened and strengthened hydrogen bonds of the amide functionality of the backbone.

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Author information

Authors and Affiliations

  1. Institut für Analytische Chemie, Technische Universität Dresden, D-01062, Dresden, Federal Republic of Germany

    W. B. Fischer

  2. Institut für Physikalische Chemie, Universität Essen, Schützenbahn 70, D-45117, Essen, Federal Republic of Germany

    P. Wu, U. Hoffmann & H. W. Siesler

  3. Institut für Polymerforschung Dresden e. V., Hohe Strasse 6, D-01069, Dresden, Federal Republic of Germany

    K.-J. Eichhorn

  4. Physics Department, Boston University, 590 Commonwealth Ave., Boston, MA, 02215, USA

    K. Rothschild

Authors
  1. W. B. Fischer
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  2. P. Wu
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  3. U. Hoffmann
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  4. K.-J. Eichhorn
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  5. H. W. Siesler
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  6. K. Rothschild
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Editor information

Editors and Affiliations

  1. Department of Analytical Chemistry, University of Veszprém, Veszprém, Hungary

    János Mink

  2. Institute of Isotopes, Hungarian Academy of Sciences, Budapest, Hungary

    János Mink

  3. Central Research Institute of Chemistry, Hungarian Academy of Sciences, Budapest, Hungary

    Gábor Keresztury

  4. Institut für Analytische Chemie, Technische Universität Wien, Wien, Austria

    Robert Kellner

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© 1997 Springer-Verlag Wien

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Fischer, W.B., Wu, P., Hoffmann, U., Eichhorn, KJ., Siesler, H.W., Rothschild, K. (1997). Comparison of Bacteriorhodopsin Conformational Changes with Uniaxially Elongated Aliphatic Polyamide Samples by Using FT-IR Difference Spectroscopy. In: Mink, J., Keresztury, G., Kellner, R. (eds) Progress in Fourier Transform Spectroscopy. Mikrochimica Acta Supplement, vol 14. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6840-0_103

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  • DOI: https://doi.org/10.1007/978-3-7091-6840-0_103

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82931-8

  • Online ISBN: 978-3-7091-6840-0

  • eBook Packages: Springer Book Archive

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