Summary
Structural studies on rotavirus using electron cryomicroscopy and computer image analysis have permitted visualization of each shell in the triple-layered rotavirus structure. Biochemical results have aided our interpretation of the structural organization of these layers and protein interactions seen in the three-dimensional structure, and have provided a better understanding of the structure-function relationships of the rotavirus structural proteins.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chen D, Burns JW, Estes MK, Ramig RF (1989) Phenotypes of rotavirus reassortants depend upon the recipient genetic background. Proc Natl Acad Sci USA 86: 3743–3747
Chen D, Estes MK, Ramig RF (1992) Specific interactions between, rotavirus outer capsid proteins VP4 and VP7 determine expression of a cross reactive, neutralizing VP4-specific epitope. J Virol 66: 432–439
Crawford SE, Labbe M, Cohen J, Burroughs MH, Zhou Y, Estes MK (1995) Characterization of virus-like particles produced by the expression or rotavirus capsid proteins in insect cells. J Virol 68: 5945–5952
Crowther RA (1971) Procedures for three-dimensional reconstruction of spherical viruses by Fourier synthesis from electron micrographs. Phil Trans Roy Soc Lond B 261: 129–228
Dubochet J, Adrian M, Chang JJ, Homo JC, Lepault J, McDowall AW, Schultz P (1988) Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21: 129–228
Estes MK (1995) Rotaviruses and their replication. In: Fields BN, Knipe DM, Chanock RM, Hirsch MS, Melnick JL, Monath TP, Roizman B (eds) Virology, vol 2. Raven Press, New York, pp 1625–1655
Kapikian AZ, Chanock RM (1995) Rotaviruses. In: Fields BN, Knipe DM, Chanock RM, Hirsch MS, Melnick JL, Monath TP, Roizman B (eds) Virology, vol 2. Raven Press, New York, pp 1657–1708
Labbé M, Charpilienne A, Crawford SE, Estes MK, Cohen J (1991) Expression of rotavirusVP2 produces empty corelike particles. J Virol 65: 2946–2952
Lawton JA, Prasad BVV (1996) Automated software package for icosahedral virus reconstruction. J Struct Biol 116: 209–215
Olson NH, Kolatkar PR, Oliveira MA, Cheng RH, Greve JM, McClelland A, Baker TS, Rossmann MG (1993) Structure of a human rhinovirus complexed with its receptor molecule. Proc Natl Acad Sci USA 90: 507–511
Prasad BVV, Wang GJ, Clerx JPM, Chiu W (1987) Cryo-electron microscopy of spherical viruses: an application to rotavirus. Micron and Microscopica Acta 18: 327–331
Prasad BVV, Wang GJ, Clerx JPM, Chiu W (1988) Three-dimensional structure of rotavirus. J Mol Biol 199: 269–275
Prasad BVV, Burns JW, Marietta E, Estes MK, Chiu W (1990) Localization of CP4 neutralization sites in rotavirus by three-dimensional cryo-electron microscopy. Nature (London) 343: 476–479
Prasad BVV, Chiu W (1994) Structure of rotavirus. In: Ramig RF (ed) Rotaviruses. Springer, Berlin, pp 9–29
Prasad BW, Rothnagel R, Zeng CQY, Lawton JA, Chiu W, Estes MK (1995) High resolution difference imaging of baculovirus-expressed subassemblies of rotaviral proteins and the native double-shelled particles (Abstract). Fifth international symposium on double-stranded RNA viruses. March 19-23, Jerba, Tunisia
Shaw AL, Rothnagel R, Chen D, Ramig RF, Chiu W, Prasad BVV (1993) Three-dimensional visualization of the rotavirus hemagglutinin structure. Cell 74: 693–701
Unwin PNT, Ennis PD (1984) Two configurations of a channel-forming membrane protein. Nature (London) 307: 609–613
Wang GJ, Hewlett M, Chiu W (1991) Structural variation of La Crosse virions under different chemical and physical conditions. Virology 184: 455–459
Wang G, Porta C, Chen Z, Baker T, Johnson JE (1992) Identification of a Fab interaction footprint site on an icosahedral virus by cryoelectron microscopy and X-ray crystallography. Nature 355: 275–278
Yeager M, Dryden KA, Olson NH, Baker TS (1990) Three-dimensional structure of rhesus rotavirus by cryoelectron microscopy and image reconstruction. J Cell Biol 110: 2133–2144
Yeager M, Berriman JA, Baker TS, Bellamy AR (1994) Three-dimensional structure of the rotavirus haemagglutinin VP4 by cryo-electron microscopy and difference map analysis. EMBO J 13: 1011–1018
Zeng Q, Labbe M, Cohen J, Prasad BVV, Chen D, Ramig RF, Estes MK (1994) Characterization of rotavirus VP2 particles. Virology 201: 55–65
Prasad BVV, Rothnagel R, Zeng CQ-Y, Jakana J, Lawton JA, Chiu W, Estes MK (1996) Visualization of ordered genomic RNA and transcriptional complexes in rotavirus. Nature 382: 471–473
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag Wien
About this paper
Cite this paper
Shaw, A.L. et al. (1996). Rotavirus structure: interactions between the structural proteins. In: Chiba, S., Estes, M.K., Nakata, S., Calisher, C.H. (eds) Viral Gastroenteritis. Archives of Virology, vol 12. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6553-9_3
Download citation
DOI: https://doi.org/10.1007/978-3-7091-6553-9_3
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82875-5
Online ISBN: 978-3-7091-6553-9
eBook Packages: Springer Book Archive