Summary
The aim of this study was to investigate the possibility of mesencephalic progenitors (MP) in treating Parkinson’s disease (PD). MP were prepared from E11–13 rats and proliferated in serum-free medium with basic fibroblast growth factor (bFGF) for 10 days. Cells were then collected and implanted into the striatum only — single grafts or simultaneously into the substantia nigra (SN) and the striatum — double grafts. Twelve weeks after transplantation, DiI, a fluorescent dye, was microinjected into the ipsilateral striatum. Using this strategy, it was found that MP of double grafts had more potent effects on rotational behavior than that of single grafts. Injection of the retrograde tracer DiI into the striatum resulted in fluorescent-labeled cells within the intranigral grafts in double grafts. These data greatly support that MP transplants could not only improve rotational behavior, but does help to re-establish nigrostriatal connections so that it may become one efficient way in treating PD.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bjorklund A (2000) Cell replacement strategies for neurodegenerative disorders. Novartis Found Symp 231: 7–15; discussion 16–20 (review)
Carvey PM, Ling ZD, Sortwell CEA (2001) Clonal line of mesencephalic progenitor cells converted to dopamine neurons by hematopoietic cytokines: a source of cells for transplantation in Parkinson’s disease. Exp Neurol 172(1): 98–108
Honig MG, Hume RI (1986) Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in longterm cultures. J Cell Biology 103: 171–187
Mendez I, Sadi D, Hong M (1996) Reconstruction of nigrostriatal pathway by simultaneous intrastriatal and intranigral DAergic transplants. J Neurosci 16(22): 7216–7227
Moore AE, Cicchtti F, Hennen J, Isacson O (2001) Parkinsonian motor deficits are reflected by proportional A9/A10 dopamine neuron degeneration in the rat. Exp Neurol 172(2): 363–376
Paxinos G, Watson C (1982) The rat brain in stereotaxic coordinates. Academic Press, London
Ptak LR, Hart KR, Lin D, Carvey PM (1995) Isolation and manipulation of rostral mesencephalic tegmental progenitor cells from rat. Cell Transplant 4(3): 335–342
Rina SS, Jauniaux E, Stern GM, Bradford HF (2002) The controlled conversion of human neural progenitor cells derived from foetal ventral mesencephalon into dopaminergic neurons in vitro. Brain Res DevBrain Res 30: 136(1); 27–34
Son JH, Chun HS, Joh TH, Cho S, Conti B, Lee JW (1999) Neuroprotection and neuronal differentiation studies using substantia nigra dopaminergic cells derived from transgenic mouse embryos. J Neurosci 19(1): 10–20
Zhou FC, Chiang YH, Wang Y (1996) Constructing a new nigrostriatal pathway in the parkinsonian model with bridged neural transplantation in substantia nigra. J Neurosci 16: 6965–6974
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer-Verlag Wien
About this paper
Cite this paper
Sun, Z.H. et al. (2003). Mesencephalic progenitors can improve rotational behavior and reconstruct nigrostriatal pathway in PD rats. In: Katayama, Y. (eds) Neurosurgical Re-Engineering of the Damaged Brain and Spinal Cord. Acta Neurochirurgica Supplements, vol 87. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6081-7_36
Download citation
DOI: https://doi.org/10.1007/978-3-7091-6081-7_36
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-7223-0
Online ISBN: 978-3-7091-6081-7
eBook Packages: Springer Book Archive