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Lipopolysaccharide Core Oligosaccharide Biosynthesis and Assembly

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Bacterial Lipopolysaccharides

Abstract

Gram-negative bacteria express on their outer membrane lipopolysaccharide (LPS) that typically comprises of three structural components: lipid A, core oligosaccharide and the O-polysaccharide (OPS). The biosynthesis, on the other hand, takes place at the cytoplasmic face of the inner membrane via two separate pathways for lipid A-core oligosaccharide and OPS that converge physically in the periplasmic face of the inner membrane. There, the undecaprenyl-diphosphate-carried OPS is joined by a carbon-oxygen ligase onto lipid A core and the resulting completed LPS molecule is shuffled onto outer membrane by the recently delineated Lpt translocation pathway.

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Authors and Affiliations

  1. Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/4c, D-23845, Borstel, Germany

    Uwe Mamat

  2. Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, 21, Haartmaninkatu 3, FIN-00014, Helsinki, Finland

    Mikael Skurnik

  3. Laboratory Microbial Pathogenesis, Consejo Superior Investigaciones Científicas, Fundación de Investigación Sanitaria Illes Balears, Recinto Hospital Joan March, Carretera Sóller Km12, 07110, Bunyola, Spain

    José Antonio Bengoechea

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  1. Uwe Mamat
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  2. Mikael Skurnik
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  3. José Antonio Bengoechea
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  1. N.D. Zelinsky Institute of Organic Chemi, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow, V-334, 119991, Russian Federation

    Yuriy A. Knirel

  2. Centre for Human Immunology and, Department of Microbiology and Immunolog, University of Western Ontario, London, N6A 5C1, Ontario, Canada

    Miguel A. Valvano

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Mamat, U., Skurnik, M., Bengoechea, J.A. (2011). Lipopolysaccharide Core Oligosaccharide Biosynthesis and Assembly. In: Knirel, Y., Valvano, M. (eds) Bacterial Lipopolysaccharides. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0733-1_8

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