Abstract
To investigate the effects of biofortification of activated sludge reactors using genetically engineered microbes containing a plasmid encoding atrazine chlorohydrolase, we compared biodegradation efficiency and sludge properties during three operating stages in a conventional activated sludge (CAS) reactor and a MBR. Our results show that with the addition of genetically engineered bacteria and the selection for indigenous biodegrading microbes, membrane fouling was reduced. Atrazine has a certain level of biotoxicity toward activated sludge and shows an inhibitory effect toward pollutant removal. After biofortification, atrazine degradation was superior in MBR than that in CAS reactors. The atrazine removal rate was 92.6 % in the MBR, while it was 82.6 % in the CAS reactor. Following biofortification, the sludge concentration in the MBR was maintained at 7.3 g/L, while the sludge concentration in the CAS reactor was 2.3 g/L.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Meclure NC, Weightman AJ et al (1989) Survival of pseudomonas putida UWC1 containing cloned catabolic genes in a model activated sludge unit. Appl Environ Microbiol 55:2627–2634
Fujita M, Ike M et al (1991) Feasibility of wastewater treatment using genetically engineered microorganisms. Water Res 25(8):979–984
Fujita M, Ike M et al (1993) Accelerated phenol removal by amplifying the gene expression with a recombinant plasmid encoding catechol-2, 3-oxygenase. Water Res 27(1):9–13
James DB, Robert RS (1997) Retention and expression of recombinant plasmids in suspended and biofilm bound bacteria degrading trichloroethene (TCE). Water Sci Technol 36(10):1–8
Erb RW, Wagner I et al (1997) Bioprotection of microbial communities from toxic phenol mixtures by a genetically designed pseudomonas. Nat Biotechnol 15(4):378–382
Soda S (1998) Effects of inoculation of a genetically engineered bacterium on performance and indigenous bacteria of a sequencing batch activated sludge process treating phenol. Ferment Bioeng 86(1):90–96
Eichner C (1999) A thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community. Appl Environ Microbiol 65(1):102–109
Nico B (2003) Bioaugmentation as a tool to protect the structure and function of an activated sludge microbial community against a 3-chloroaniline shock load. Appl Environ Microbiol 69(3):1511–1520
Huang X, Liu R, Qian Y (2011) Behavior of soluble microbial products in a membrane bioreactor. Process Biochem 36:401–406
Lisa SC, Hugh MT, Michael SJ et al (2010) Field-scale remediation of atrazine-contaminated soil using recombinant Escherichia coli expressing atrazine chlorohydrolase. Environ Microbiol 2(1):91–98
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Wang, Y., Sun, J. (2015). Biofortification Using Bacteria Containing an Atrazine-Degrading Gene and Its Effects on Reactor Operating Efficiency. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_47
Download citation
DOI: https://doi.org/10.1007/978-3-662-46318-5_47
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-46317-8
Online ISBN: 978-3-662-46318-5
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)