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Regulation of Collagen IV Expression

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Nephrology

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Summary

Recent advances in the molecular biology of basement membrane components have resulted in new tools for the study of basement membrane metabolism during normal renal development and growth. Collagen IV, the major structural protein of basement membranes, is assembled from at least 5 distinct gene products. mRNA encoding the α1(IV) and α2(IV) chains vary in a precise temporal and spatial fashion during nephronogenesis. Inflammation, such as that observed in antiglomerular basement membrane (GBM) nephritis or in the metabolic or hemodynamic disturbances exemplified by diabetes mellitus, leads to increased steady-state levels of mRNA for these basement membrane components. Increased transcription of these genes may, in part, account for the increase. These two genes are separated by a 130 bp bidirectional promoter which is regulated by sequences contained within the genes. We have directly measured transcription of each gene by the nuclear run-on method and have correlated this with the steady-state mRNA level for each chain. The data suggest that transcriptional attenuation may play an important role in determining the ratio of α1(IV)/α2(IV) mRNA as well as the relative abundance of these mRNA in different tissues. These methods should provide new insights into the mechanism of collagen IV gene expression during renal development and in experimental disease models.

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References

  1. Ekblom P, Vestweber D, Kemler R (1986) Cell-matrix interactions and cell adhesion during development. Annu Rev Cell Biol 2: 27–47

    Article  PubMed  CAS  Google Scholar 

  2. Sugrue SP, Hay ED (1986) The identification of extracellular matrix ( ECM) binding sites on the basal surface of embryonic corneal epithelium and the effect of ECM binding on epithelial collagen production. J Cell Biol 102: 1907–1916

    Google Scholar 

  3. Vracko, R (1974) Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure. Am J Pathol 77: 314–346

    PubMed  CAS  Google Scholar 

  4. Maddox DA, Brenner BM (1977) Glomerular filtration of fluid and macromolecules: the renal response to injury. Annu Rev Med 28: 91–102

    Article  PubMed  CAS  Google Scholar 

  5. Saus J, Wieslander J, Langveld JP, Quinones S, Hudson BG (1988) Identification of the Goodpasture antigen as the a3(IV) chain of collagen IV. J Biol Chem 263: 13374–13380

    PubMed  CAS  Google Scholar 

  6. Kleppel MM, Santi PA, Cameron JD, Wieslander J, Michael AF (1989) Human tissue distribution of novel basement membrane collagen. Am J Pathol 134: 813–825

    PubMed  CAS  Google Scholar 

  7. Hostikka S, Eddy RL, Byers MG, Hoyhtya M, Shows TB, Tryggvason K (1990) Identification of a distinct type IV collagen alpha chain with restricted kidney distribution and assignment of its gene to the locus of X chromosome-linked Alport syndrome. Proc Natl Acad Sci USA 87: 1606–1610

    Article  PubMed  CAS  Google Scholar 

  8. Kleinman HK, Ebihara I, Killen PD, Sasaki M, Cannon FB, Yamada Y, Martin GR (1987) Genes for basement membrane proteins are coordinately expressed in differentiating F9 cells but not in normal adult murine tissues. Dev Biol 122: 373–378

    Article  PubMed  CAS  Google Scholar 

  9. Boot-Hanford RP, Kurkinen M, Prockop DJ (1987) Steady-state levels of mRNAs for the type IV collagen and laminin polypeptide chains of basement membranes exhibit marked tissue-specific stoichiometric variations in the rat. J Biol Chem 262: 12475–12478

    Google Scholar 

  10. Burbelo PD, Yamada Y, Martin GR (1988) Alpha 1(IV) and alpha2(IV) collagen genes are regulated by a bidirectional promoter and a shared enhancer. Proc Natl Acad Sci USA 85: 9679–9682

    Article  PubMed  CAS  Google Scholar 

  11. Poschl E, Pollner R, Kuhn K (1988) The genes for the alpha 1 (IV) and alpha 2(IV) chains of human basement membrane collagen IV are arranged head-to-head and separated by a bidirectional promoter of unique structure. EMBO J 7: 2687–2695

    PubMed  CAS  Google Scholar 

  12. Cleveland DW, Lopata MA, McDonald RI, Cowan NJ, Rutter WJ (1980) Number and evolutionary conservation of a-and 13-tubulin and cytoplasmic ß-and y-actin genes using specific cloned cDNA probes. Cell 20: 95–105

    Article  PubMed  CAS  Google Scholar 

  13. Feinberg A, Vogelstein B (1982) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13

    Article  Google Scholar 

  14. McKnight GS, Palmiter RD (1979) Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct. J Biol Chem 254: 9050–9058

    PubMed  CAS  Google Scholar 

  15. Chan YL, Olvera J, Wool IG (1983) Structure of rat 28S ribosomal ribonucleic acid inferred from the sequence of nucleotides in a gene. Nucleic Acids Res 11: 7819–7831

    Article  PubMed  CAS  Google Scholar 

  16. Oberbaumer I, Magdolen U (1988) The 3’ untranslated region of the murine mRNA for the alpha 2 chain of type IV collagen contains a BI-like element. Nucleic Acids Res 16: 7181

    Article  PubMed  CAS  Google Scholar 

  17. Ebihara I, Killen PD, Laurie GW, Huang T, Yamada Y, Martin GR, Brown KS (1988) Altered mRNA expression of basement membrane components in a murine model of poly-cystic kidney disease. Lab Invest 58: 262–269

    PubMed  CAS  Google Scholar 

  18. Laurie GW, Horikoshi S, Killen PD, Segui-Real B, Yamada Y (1989) In situ hybridization reveals temporal and spatial changes in cellular expression of mRNA for a laminin receptor, laminin, and basement membrane (type IV) collagen in the developing kidney. J Cell Biol 109: 1351–1362

    Article  PubMed  CAS  Google Scholar 

  19. Merrit S, Killen PD, Wiggins R (to be published) Analysis of a 1(I) procollagen, a I (IV) collagen ß and [3-actin mRNA in glomeruli and cortex of rabbits with experimental anti-GBM disease. Evidence of early extraglomerular collagen biosynthesis. Lab Invest

    Google Scholar 

  20. Bender TP, Thompson CB, Kuehl WM (1987) Differential expression of c-myb mRNA in murine B lymphomas by a block to transcription elongation. Science 237: 1473–1476

    Article  PubMed  CAS  Google Scholar 

  21. Lindstein T, June CH, Thompson CB (1988) Multiple mechanisms regulate c-myc expression during normal T cell activation. EMBO J 7: 2787–2794

    Google Scholar 

  22. Lindsten T, June CH, Thompson CB, Leiden JM (1988) Regulation of 4F2 heavy-chain gene expression during normal human T-cell activation can be mediated by multiple distinct molecular mechanisms. Mol Cell Biol 8: 3820–3826

    PubMed  CAS  Google Scholar 

  23. Killen PD, Burbelo PD, Martin GR, Yamada Y (1988) Characterization of the promoter of the a1(IV) collagen gene. J Biol Chem 263: 12310–12314

    PubMed  CAS  Google Scholar 

  24. Killen PD, DeMeester CA, Long R, O’Brien E (1991) Identification of sequences which enhance or repress collagen IV gene transcription. Proceedings of the XIth International Congress of Nephrology, July 15–20 1990. Tokyo, Japan

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, School of Medicine, University of Michigan, Ann Arbor, MI, 48109-0602, USA

    Joseph P. Grande

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  1. Paul D. Killen
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  2. Cynthia A. DeMeester
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  3. Robert A. Long
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  4. Erin O’Brien
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  5. Joseph P. Grande
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Editor information

Editors and Affiliations

  1. Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan

    Michinobu Hatano M.D. (Professor of Medicine, Director) (Professor of Medicine, Director)

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© 1991 Springer Japan

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Killen, P.D., DeMeester, C.A., Long, R.A., O’Brien, E., Grande, J.P. (1991). Regulation of Collagen IV Expression. In: Hatano, M. (eds) Nephrology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-35158-1_116

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  • DOI: https://doi.org/10.1007/978-3-662-35158-1_116

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-70074-6

  • Online ISBN: 978-3-662-35158-1

  • eBook Packages: Springer Book Archive

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